Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Hematologists commonly employ both microscopy and flow cytometry to study and classify blood cells, normal and abnormal hematopoesis, and hematopathologies. Amnis® imaging flow cytometry's unique ability to quantify images of large numbers of cells in suspension makes the technology ideally suited for the field of Hematology. Some example applications include combined immunophenotypic and morphology-based classification of the different stages of erythropoiesis, identification of sickle cells or parasite-infected RBC, and quantification of phagocytosis, co-localization, chemokine-induced shape change, and cell signaling within specific subsets of circulating blood cells.
As hematopoeitc stem cells differentiate through the erythroid lineage, they shrink and eventually enucleate as they progress towards becoming RBC. They also progressively gain expression of glycophorinA and lose CD71 expression. The extruded nuclei express low levels of glycophorinA on their membranes. This experiment demonstrates the unique ability of the Imagestream®x to classify cells using a combination of intensity, morphology, and location based parameters.
Hematologists commonly employ both microscopy and flow cytometry to study and classify blood cells, normal and abnormal hematopoesis, and hematopathologies. Amnis® imaging flow cytometry's unique ability to quantify images of large numbers of cells in suspension makes the technology ideally suited for the field of Hematology. Some example applications include combined immunophenotypic and morphology-based classification of the different stages of erythropoiesis, identification of sickle cells or parasite-infected RBC, and quantification of phagocytosis, co-localization, chemokine-induced shape change, and cell signaling within specific subsets of circulating blood cells.