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101167 Amido black 10 B (C.I.20470)

for electrophoresis

Amido black 10 B (C.I.20470) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Black acid 1, Amido black 10 B (C.I. 20470), Naphthol blue black

CAS #: 1064-48-8 EC Number: 213-903-1 Molar Mass: 616.5 g/mol Hill Formula: C₂₂H₁₄N₈Na₂O₉S₂
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101167
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Overview

Replacement Information

Key Spec Table

CAS #EC NumberHill FormulaMolar Mass
1064-48-8213-903-1C₂₂H₁₄N₈Na₂O₉S₂616.5 g/mol
Description
Catalogue Number101167
SynonymsBlack acid 1, Amido black 10 B (C.I. 20470), Naphthol blue black
DescriptionAmido black 10 B (C.I.20470)
OverviewAmido black 10 B (C.I.20470) for electrophoresis, is used for staining connective tissue, in electrophoresis, and as a redox indicator. Proteins possess free amino and carboxyl groups and are charged positively or negatively in aqueous solutions, depending on the pH. In an alkaline milieu they are negatively charged and migrate in the electric field towards the anode at a speed that depends on the number of charges they carry. This difference in migration velocities makes it possible to separate the serum protein mixture into groups of individual components.The separated protein fractions are stained with amido black and measured photometrically or densitometrically. As a rule, five fractions are observed after staining - albumin, alpha 1- and alpha 2-globulins, beta 1- and beta 2-globulins, and beta-globulins - where albumin has the highest migration velocity and gamma-globulin the lowest. Differentiation of serum proteins by electrophoresis is important for the diagnosis and prognosis of numerous diseases. Changes in protein composition can be found e. g. in inflammatory diseases, tumors, liver diseases, antibody deficiency syndromes or disturbances in protein balance. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
CAS number1064-48-8
EC number213-903-1
Hill FormulaC₂₂H₁₄N₈Na₂O₉S₂
Molar Mass616.5 g/mol
HS Code3212 90 00
Structure formula ImageStructure formula Image
Quality LevelMQ400
Applications
Applicationfor electrophoresis
Biological Information
Physicochemical Information
pH value9.0 (10 g/l, H₂O, 25 °C)
Bulk density670 kg/m3
Solubility10 g/l
Dimensions
Materials Information
Toxicological Information
LD 50 oralLD50 Rat > 5000 mg/kg
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Dye content (spectrophotometrically)≥ 80 %
Identity
- sodium
passes test
- UV/VIS-Spectrumpasses test
Absorption maximum λ max (water)617 - 620 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water)750 - 960
TLC-Testpasses test
Loss on drying (110 °C)≤ 10 %
Suitability for electrophoresispasses test
Suitability as redox indicatorpasses test