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IM42L Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6)

Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MMP-14

IM42L
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      OverviewRecognizes the ~60-66 kDa MTI-MMP protein in HT-1080 cells and ConA-treated MD-MB-231 cells.
      Catalogue NumberIM42L
      Brand Family Calbiochem®
      SynonymsAnti-MMP-14
      References
      ReferencesMattei, M.G., et al. 1997. Genomics 40, 168. Okada, A., et al. 1997. J. Cell. Biol. 147, 67. Okumura, Y., et al. 1997. FEBS Lett. 402, 181. Ueno, H., et al. 1997. Cancer Res. 57, 2055. Strongin, A.Y., et al. 1995. J. Biol. Chem. 270, 5331. Takino, T., et al. 1995. J. Biol. Chem. 270, 23013. Sato, H., et al. 1994. Nature 370, 61. Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861. Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434. Fridman, R., et al. 1992. J. Biol. Chem. 267, 15389. Woessner, J.F. 1991. FASEB J. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106. Goldberg, G.I., et al. 1989. PNAS 86, 8207.
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controltrpE (Ab-1) or untreated MD-MB-231 cells
      Positive controlHT-1080 cells, ConA-treated MD-MB-231 cells, or breast carcinoma tissue
      PreservativeNone
      Applications
      Application ReferencesImmunoblotting, Paraffin Sections, Immunocytochemistry Ueno, H., et al. 1997. Cancer Res. 57, 2055.
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Not Frozen Sections
      Not Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (not recommended) Paraffin Sections (10 µg/ml, Pronase™ or heat pre-treatment required) Immunoblotting (10 µg/ml) Immunocytochemistry (see application reference) Immunoprecipitation (not recommended)
      Application CommentsDoes not cross-react with MT2-MMP or MT3-MMP. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. For best results with paraffin sections, fix with perioddate-lysine-4% paraformaldehyde. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (REVPYAYIREGHEK) corresponding to amino acids 160-173 in the catalytic domain of human MT1-MMP
      ImmunogenHuman
      Clone114-6G6
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage REFRIGERATOR (+4°C)
      Do not freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for storage of reconstituted product for longer than a month; repetitive freezing and thawing should be avoided.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM42L 0

      Documentation

      References

      Reference overview
      Mattei, M.G., et al. 1997. Genomics 40, 168. Okada, A., et al. 1997. J. Cell. Biol. 147, 67. Okumura, Y., et al. 1997. FEBS Lett. 402, 181. Ueno, H., et al. 1997. Cancer Res. 57, 2055. Strongin, A.Y., et al. 1995. J. Biol. Chem. 270, 5331. Takino, T., et al. 1995. J. Biol. Chem. 270, 23013. Sato, H., et al. 1994. Nature 370, 61. Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861. Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434. Fridman, R., et al. 1992. J. Biol. Chem. 267, 15389. Woessner, J.F. 1991. FASEB J. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106. Goldberg, G.I., et al. 1989. PNAS 86, 8207.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-MMP-14
      ApplicationFrozen Sections (not recommended) Paraffin Sections (10 µg/ml, Pronase™ or heat pre-treatment required) Immunoblotting (10 µg/ml) Immunocytochemistry (see application reference) Immunoprecipitation (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~66 kDa latent and the ~60 kDa active forms of MT1-MMP protein.
      BackgroundMatrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components. Of the sixteen proteins reported to date, ten are normally found as soluble molecules. Several of the MMP proteins have been shown to be integral membrane proteins and have been named MT-MMP's for membrane bound MMP. The MT-MMP family is now known to contain at least three members, MT1-MMP, MT2-MMP and MT3-MMP also known as MMP-14, MMP-15 and MMP-16 respectively. While each of these proteins contain a C-terminal transmembrane domain allowing localization to the cell surface they are independently expressed. These proteins are also unique from the other members of the MMP family in that they contain an 8 amino acid insert in the catalytic domain. The MT1-MMP protein is encoded by a 4.5 kb mRNA species giving rise to a protein with a molecular weight of 60 66 kDa by SDS-PAGE. MT1-MMP is responsible for cleaving progelatinase A (MMP-2, 72 kDa Type IV collagenase) and progelatinase B to their active forms. MT1-MMP itself requires an activation step which is the result of activity of the membrane plasmin cascade. MT1-MMP functions by binding TIMP-2 and then the COOH terminal end of MMP-2 resulting in a 105 kDa trimer which effects the cleavage of pro-MMP-2 to the biologically active form. The order of the binding of pro-MMP-2 and TIMP-2 to MT1-MMP is critical as TIMP-2 will also inhibit the activity of MMP-2 when present in a soluble form.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide (REVPYAYIREGHEK) corresponding to amino acids 160-173 in the catalytic domain of human MT1-MMP
      Clone114-6G6
      IsotypeIgG₁
      Specieshuman
      Positive controlHT-1080 cells, ConA-treated MD-MB-231 cells, or breast carcinoma tissue
      Negative controltrpE (Ab-1) or untreated MD-MB-231 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsDoes not cross-react with MT2-MMP or MT3-MMP. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. For best results with paraffin sections, fix with perioddate-lysine-4% paraformaldehyde. Antibody should be titrated for optimal results in individual systems.
      Storage REFRIGERATOR (+4°C)
      Do Not Freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for storage of reconstituted product for longer than a month; repetitive freezing and thawing should be avoided.
      Toxicity Standard Handling
      ReferencesMattei, M.G., et al. 1997. Genomics 40, 168. Okada, A., et al. 1997. J. Cell. Biol. 147, 67. Okumura, Y., et al. 1997. FEBS Lett. 402, 181. Ueno, H., et al. 1997. Cancer Res. 57, 2055. Strongin, A.Y., et al. 1995. J. Biol. Chem. 270, 5331. Takino, T., et al. 1995. J. Biol. Chem. 270, 23013. Sato, H., et al. 1994. Nature 370, 61. Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861. Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434. Fridman, R., et al. 1992. J. Biol. Chem. 267, 15389. Woessner, J.F. 1991. FASEB J. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106. Goldberg, G.I., et al. 1989. PNAS 86, 8207.
      Application referencesImmunoblotting, Paraffin Sections, Immunocytochemistry Ueno, H., et al. 1997. Cancer Res. 57, 2055.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies