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MABE1066-100UG Anti-SETDB1 Antibody, clone F0838

Anti-SETDB1, clone F0838, Cat. No. MABE1066, is a highly specific mouse monoclonal antibody that detects SETDB1 and has been tested for use in Western Blotting.

Anti-SETDB1 Antibody, clone F0838 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Setdb1, Eset, Kiaa0067 UniProt Number: O88974
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MABE1066-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1066-100UG
      DescriptionAnti-SETDB1 Antibody, clone F0838
      Alternate Names
      • Histone-lysine N-methyltransferase SETDB1
      • EC: 2.1.1.43
      • ERG-associated protein with SET domain
      • ESET
      • SET domain bifurcated 1
      Background InformationHistone-lysine N-methyltransferase SETDB1 (UniProt: O88974; also known as EC: 2.1.1.43, ERG-associated protein with SET domain, ESET, SET domain bifurcated 1) is encoded by the Setdb1 (also known as Eset, Kiaa0067) gene in human. SETDB1 is ubiquitously expressed, but a strong expression is observed in liver and testis. It is a histone methyltransferase that specifically trimethylates Histone 3 Lysine 9 (H3K9). H3K9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. SETDB1 is reported to inhibit adipogenesis. It has also been shown that H3K4/H3K9me3 bivalent chromatin signature keeps expression of Cebpa and Pparg genes very low. Depletion of SETDB1 can induce adipogenesis in mesenchymal stem cells and in preadipocytes. SETDB1 contains two Tudor domains (aa 257-320 and 347-403) and one MBD domain (aa 611-682). It also has a SET domain (aa 820-1282), a pre-SET (aa 744-817), and a post-SET domain (aa 1291-1307). The pre-SET, SET and post-SET domains are required for its methyltransferase activity. In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster. (Ref.: Matsumura, Y., et al., (2015), Mol. Cell 60(4); 584- 596).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
      Applications
      ApplicationAnti-SETDB1, clone F0838, Cat. No. MABE1066, is a highly specific mouse monoclonal antibody that detects SETDB1 and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected SETDB1 in Western Blotting applications (Matsumura, Y, et. al. (2015). Mol Cell. 60(4):584-96).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 213 amino acids from the N-terminal half of murine Histone-lysine N-methyltransferase SETDB1.
      CloneF0838
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone F0838 recognizes SETDB1 in murine embryonic stem cells. It targets an epitope with in 213 amino acids from the N-terminal half of isoform 1 and 7.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Setdb1
      • Eset
      • Kiaa0067
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~160 and 180 kDa observed; 144.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in 129/S6/SvEv mouse embryonic stem cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected SETDB1 in 129/S6/SvEv mouse embryonic stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1066-100UG 04054839540202