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2114 Detergent-OUT™ Micro Kit

The Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions.

Detergent-OUT™ Micro Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Protein Clean-up
2114
1 kit  10 columns
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      PCU
      Description
      Catalogue Number2114
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDetergent-OUT™ Micro Kit
      OverviewThe Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. Micro columns are suitable for removing detergent from a maximum of 0.2 mL sample volume.
      Materials Required but Not DeliveredCentrifuge

      Centrifuge tubes
      References
      Product Information
      Components
      • Detergent-OUT Columns-DTG-100X 10 Micro
      • Collection Tubes (2 mL) 10
      Applications
      ApplicationThe Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions.
      Key Applications
      • Protein Clean-up
      Application NotesPROTOCOL:

      1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.

      2. Buffer Equilibration: SpinOUT columns are supplied in deionized water. Equilibrate the column with a buffer of your choice, as follows. Note: Do not equilibrate the columns with Tris buffer. Apply about 0.5 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.

      3. Place the column in a 2.0 mL micro-centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.

      4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 1-2 minutes. Note: If your protein solution is in Tris buffer, exchange the protein solution buffer to phosphate or other buffer, using either a SpinOUT column or by dialysis.

      5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
      Packaging Information
      Material Size1 kit
      Material Package10 columns
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      2114 04053252278426

      Documentation

      Detergent-OUT™ Micro Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Detergent-OUT™ Micro Kit Certificates of Analysis

      TitleLot Number
      DETERGENT-OUT KIT (MICRO) - 1984691 1984691
      DETERGENT-OUT KIT (MICRO) - 2058351 2058351
      DETERGENT-OUT KIT (MICRO) - 3170442 3170442
      DETERGENT-OUT KIT (MICRO) - 3194175 3194175
      DETERGENT-OUT KIT (MICRO) - 3253471 3253471
      DETERGENT-OUT KIT (MICRO) - 3279774 3279774
      DETERGENT-OUT KIT (MICRO) - 3291975 3291975
      DETERGENT-OUT KIT (MICRO) - 3305000 3305000
      DETERGENT-OUT KIT (MICRO) - 3386072 3386072
      DETERGENT-OUT KIT (MICRO) - 3446869 3446869
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      Data Sheet

      Title
      DETERGENT-OUT KIT (MICRO)