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109204 Giemsa's azur eosin methylene blue solution

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109204
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1092040100
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      Glass bottle 100 ml
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      1092040500
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          Plastic bottle 500 ml
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          1092041000
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              Plastic bottle 1 l
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              1092042500
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                  Plastic bottle 2.5 l
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                  Description
                  Catalogue Number109204
                  DescriptionGiemsa's azur-eosin-methylene blue solution
                  OverviewGiemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
                  References
                  Product Information
                  HS Code3212 90 90
                  Quality LevelMQ400
                  Applications
                  Applicationfor microscopy
                  Biological Information
                  Physicochemical Information
                  Boiling point>65 °C (1013 hPa)
                  Density0.99 g/cm3 (20 °C)
                  Explosion limit5.5 - 44 %(V) Methanol
                  Flash point18 °C
                  Ignition temperature455 °C
                  pH value6.0 - 8.2 (H₂O, 20 °C) (undiluted)
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Hazard Pictogram(s) Hazard Pictogram(s)
                  Hazard Pictogram(s)
                  Hazard Pictogram(s)
                  Hazard Statement(s)H225: Highly flammable liquid and vapour.
                  H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
                  H317: May cause an allergic skin reaction.
                  H370: Causes damage to organs.
                  Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
                  P233: Keep container tightly closed.
                  P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
                  P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
                  P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
                  P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
                  P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
                  Signal WordDanger
                  Storage class3 Flammable liquids
                  WGKWGK 2 obviously hazardous to water
                  Disposal9
                  Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
                  Safety Information
                  Categories of dangerhighly flammable, toxic
                  Product Usage Statements
                  Storage and Shipping Information
                  StorageStore at +15°C to +25°C.
                  Packaging Information
                  Transport Information
                  Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
                  Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
                  Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
                  Supplemental Information
                  Specifications
                  Suitability for microscopypasses test
                  Erythrocytesreddish-brownish
                  Nuclei / Chromatinred to violet
                  Eosinophilic granulesreddish to red-brown
                  Neutrophilic granuleslight violet
                  Lymphocyte cytoplasmnblue
                  Monocyte cytoplasmgrey-blue
                  Thrombocytesviolet
                  Basophilic granulesdark violet
                  Nuclei (Tissue section)blue to dark blue
                  Cellsblue to dark blue
                  Collagenpale blue
                  Osteoidpale blue
                  Eosinophilic granula (Tissue section)red
                  Acidic mucopolysaccharidesreddish-violet
                  Mast cell granulesreddish-violet
                  Cartilage matrixreddish-violet
                  Acidophilic materialsorange-red
                  Helicobacter pylori
                  blue to dark blue
                  blue to dark blue
                  Global Trade Item Number
                  Catalogue Number GTIN
                  1092040100 04022536111841
                  1092040500 04022536111889
                  1092041000 04022536720081
                  1092042500 04022536111926

                  Documentation

                  Giemsa's azur eosin methylene blue solution SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Giemsa's azur eosin methylene blue solution Certificates of Analysis

                  Product Number PackagingSpecificationLot Number
                  1092040100 Glass bottlePDF Specification Document
                  1092040500 Plastic bottlePDF Specification Document
                  1092041000 Plastic bottlePDF Specification Document
                  1092042500 Plastic bottlePDF Specification Document

                  User Guides

                  Title
                  User Guide (IFU) - 109204

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                  Categories

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