17-500M
Catch and Release v2.0 Reversible Immunoprecipitation System
別名:
Catch and release system, Immunoprecipitation kit
species reactivity (predicted by homology)
all
manufacturer/tradename
Catch And Release
Upstate®
technique(s)
activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
wet ice
Quality Level
Analysis Note
routinely evaluated by immunoprecipitation.
Application
Catch and Release v2.0 should not be used with proteins containing a 6-His tag.
General description
Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Other Notes
Catch and Release Wash Buffer, 10X
Catch and Release Non-denaturing Elution Buffer, 4X
Catch and Release Denaturing Elution Buffer, 1X
Catch and Release Capture Tubes
Catch and Release Non-denaturing Elution Buffer, 4X
Catch and Release Denaturing Elution Buffer, 1X
Catch and Release Capture Tubes
Packaging
17-500A is supplied as 5 immunoprecipitations per kit.
17-500 is supplied as 50 immunoprecipitations per kit.
17-500 is supplied as 50 immunoprecipitations per kit.
Preparation Note
All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
保管分類
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Please refer to KIT Component information
pdsc
Please refer to KIT Component information
prtr
Please refer to KIT Component information
fsl
Please refer to KIT Component information
ishl_indicated
Please refer to KIT Component information
ishl_notified
Please refer to KIT Component information
cart
キットコンポーネントの情報を参照してください
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Neonatal hypoxia-ischemia differentially upregulates MAGUKs and associated proteins in PSD-93-deficient mouse brain
Jiang, X., et al
Stroke, 34, 2958-2963 (2003)
Nan Zhang et al.
Diabetes, 53(4), 963-970 (2004-03-30)
Successful islet transplantation depends on the infusion of sufficiently large quantities of islets, of which only approximately 30% become stably engrafted. Rapid and adequate revascularization of transplanted islets is important for islet survival and function. Delayed and insufficient revascularization can
Hypercholesterolemia-induced Abeta accumulation in rabbit brain is associated with alteration in IGF-1 signaling.
Sharma, Sunita, et al.
Neurobiology of Disease, 32, 426-432 (2008)
Wanxia He et al.
Journal of virology, 77(19), 10566-10574 (2003-09-13)
Addition of human papillomavirus (HPV) E7 CDK2/cyclin A or CDK2/cyclin E, purified from either insect cells or bacteria, dramatically upregulates histone H1 kinase activity. Activation is substrate specific, with a smaller effect noted for retinoblastoma protein (Rb). The CDK2 stimulatory
Enhanced protein recovery and reproducibility from pull-down assays and immunoprecipitations using spin columns.
A Brymora et al.
Analytical biochemistry, 295(1), 119-122 (2001-07-31)
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 17-500A | 04053252744976 |
| 17-500 | 04053252323119 |
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