クロラムフェニコール

Chloramphenicol (CAP) is an antibiotic which was first isolated from Streptomyces venezuelae. It has a nitrobenzene moiety which may be responsible for aplastic anaemia.1 It is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.
This grade has the standard for Supelco MIP SPE cartridges. For more information request Supelco Literature T407075, T706024

Chloramphenicol has been used as reference standard in the determination of the concentration of CAP residues in shrimp tissues using LC-MS technique and also in frozen chicken samples (liver, kidney and muscle) using HPLC.

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

作用機序：クロラムフェニコールは、50Sリボソームサブユニットに結合してアミノアシルtRNAのリボソームへの付着を防ぐことでペプチジルトランスフェラーゼステップを遮断することにより細菌のタンパク質合成を阻害します。また、ミトコンドリアと葉緑体のタンパク質合成およびリボソームの(p)ppGpp生成を阻害して、rRNA転写を抑制します。

耐性機序：クロラムフェニコールアセチルトランスフェラーゼを使用することで、本製品はアセチル化され、不活性化されます。

抗菌スペクトル：グラム陽性菌およびグラム陰性菌に対して幅広いスペクトルを持つ抗生物質であり、主に眼科や獣医学的用途で使用されます。

Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

VETRANAL is a registered trademark of Merck KGaA, Darmstadt, Germany

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.