T3163
テトラメチルローダミンイソチオシアナート, アイソマー R
powder
別名:
6-TRITC, テトラメチルローダミン-6-イソチオシアナート
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この商品について
実験式（ヒル表記法）:
C25H21N3O3S
CAS番号:
分子量:
443.52
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
InChI
1S/C25H21N3O3S/c1-27(2)16-6-9-19-22(12-16)31-23-13-17(28(3)4)7-10-20(23)24(19)21-11-15(26-14-32)5-8-18(21)25(29)30/h5-13H,1-4H3
SMILES string
CN(C)c1ccc2c(OC3=C\C(C=CC3=C2c4cc(ccc4C([O-])=O)N=C=S)=[N+](/C)C)c1
InChI key
OBYNJKLOYWCXEP-UHFFFAOYSA-N
description
labeling efficiency with bovine albumin >= 50%
form
powder
composition
Dye content, ≥80%
solubility
1 M NH4OH: 10 mg/mL (Faint Red to Very Dark Red to Very Dark Purple solution)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Quality Level
General description
Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.
Application
- TRITC is used to label a wide variety of biomolecules, including immunoglobulins, lectins, nucleic acids, polynucleotides, and polysaccharides for affinity, immuno-, and in situ hybridization, fluorescent probes, and flow cytometry.
- Lectins are TRITC-Iabeled reagents for the affinity staining of sections and cell monolayers to distinguish the sialomucins of salivary glands.
- TRITC-antibodies are used to identify pathogenic amoebae.
- TRITC-oligonucleotides are used in the in-situ hybridization staining of soil microorganisms.
- TRITC-Iabeled reagents have also been used as fluorescent probes of live cells.
- Flow cytometry of TRlTC-Iabeled slime mold cells has been used to study their aggregation behavior.
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
T3163-VAR: + T3163-EW: + T3163-5MG:4548173951782 + T3163-BULK: + T3163-10MG:4548173951775
jan
Conn's Biological Stains
Kiernan & Horobin
Conn?s Biological Stains (2002)
In Vitro Study of Nuclear Assembly and Nuclear
Import Using Xenopus Egg Extracts
Import Using Xenopus Egg Extracts
Douglass J Forbes
Methods in Molecular Biology (2016)
E. Harlow and D. Lane, ed.
Antibodies: A Laboratory Manual, 353-355 (1988)
Thomas E Wood et al.
Cell reports, 29(1), 187-201 (2019-10-03)
The type VI secretion system (T6SS) is crucial in interbacterial competition and is a virulence determinant of many Gram-negative bacteria. Several T6SS effectors are covalently fused to secreted T6SS structural components such as the VgrG spike for delivery into target
A simple method for determining the labeling efficiency of fluorescein isothiocyanate products.
R M McKinney et al.
Analytical biochemistry, 14(3), 421-428 (1966-03-01)
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