62197
Anti-Mouse IgG (whole molecule)−Atto 488 antibody produced in goat
~1 mg/mL
別名:
Atto 488 Anti-Mouse IgG, Goat Anti-Mouse IgG
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この商品について
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
Atto 488 conjugate
Clone:
polyclonal
Application:
immunocytochemistry
immunofluorescence
immunofluorescence
Species reactivity:
mouse
Citations:
28
Technique(s):
immunocytochemistry: 1:500
immunofluorescence: 1:400
immunofluorescence: 1:400
製品名
Anti-Mouse IgG (whole molecule)−Atto 488 antibody produced in goat, ~1 mg/mL
biological source
goat
conjugate
Atto 488 conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
liquid
contains
50% glycerol as stabilizer
species reactivity
mouse
concentration
~1 mg/mL
technique(s)
immunocytochemistry: 1:500
immunofluorescence: 1:400
fluorescence
λex 500 nm; λem 522 nm in PBS
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
関連するカテゴリー
Analysis Note
unconjugated dye ≤5% of total fluorescence
Application
Anti-Mouse IgG (whole molecule)?Atto 488 antibody produced in goat has been used:
- in immunofluorescence confocal microscopic assay for p65 localization
- in immunohistochemical analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Biochem/physiol Actions
IgG facilitates transplacental transport and participates in the secondary immune response. It has the longest serum half life. It might be linked to allergy. IgG initiates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
IgG is the most abundant antibody in human serum. It is secreted by B cells andis found in blood. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Anti-Mouse IgG (whole molecule)-Atto 488 antibody is specific for mouse IgG. The product is purified from goat anti-mouse IgG antiserum to remove all goat serum proteins, including immunoglobulins that do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity.
Immunogen
purified mouse IgG.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Physical form
Solution in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, andcontaining 5 mM sodium azide as a preservative.
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保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
62197-1ML-F: + 62197-VAR-F: + 62197-BULK-F:
jan
Carlo Sala Frigerio et al.
Cell reports, 27(4), 1293-1306 (2019-04-25)
Gene expression profiles of more than 10,000 individual microglial cells isolated from cortex and hippocampus of male and female AppNL-G-F mice over time demonstrate that progressive amyloid-β accumulation accelerates two main activated microglia states that are also present during normal
Jan Andreas Ruland et al.
Nature communications, 12(1), 6211-6211 (2021-10-29)
Ribosomal biogenesis has been studied by biochemical, genetic and electron microscopic approaches, but live cell data on the in vivo kinetics are still missing. Here we analyse the export kinetics of the large ribosomal subunit (pre-60S particle) through single NPCs
Emily J Reedich et al.
Journal of neuroscience research, 100(10), 1951-1966 (2022-07-16)
Spastic cerebral palsy (CP) is a movement disorder marked by hypertonia and hyperreflexia; the most prevalent comorbidity is pain. Since spinal nociceptive afferents contribute to both the sensation of painful stimuli as well as reflex circuits involved in movement, we
IgG subclasses and allotypes: from structure to effector functions
Vidarsson G, et al.
Frontiers in Immunology, 5(1-2), 520-520 (2014)
Arnold Garbiec et al.
Arthropod structure & development, 41(2), 165-176 (2012-02-04)
Mechanisms that underlie differentiation and diversification of the ovarian follicular epithelium in insects have been best characterized in a fruit fly, Drosophila melanogaster. Recent comparative analyses have shown that dipterans evolved a common, specific system of early patterning of their
資料
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
イムノブロッティング（ウェスタンブロット転写法）は現代のプロテオミクス研究において一般的な手法です。
プロトコル
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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