646547
トリス(2-カルボキシエチル)ホスフィン 塩酸塩 溶液
0.5 M, pH 7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
別名:
TCEP
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この商品について
Quality Level
concentration
0.5 M
refractive index
n20/D 1.367
pH
7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
density
1.041 g/mL at 25 °C
SMILES string
Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O
InChI
1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
InChI key
PBVAJRFEEOIAGW-UHFFFAOYSA-N
General description
Application
- 合成ペプチドのシステイン残基の切断
- RNA Sequential Probing of Targets（SPOT）imaging用の還元バッファー
- オリゴヌクレオチドの還元
- ミトコンドリア単離時の還元剤
Biochem/physiol Actions
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
保管分類
8A - Combustible corrosive hazardous materials
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
プロトコル
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
資料
Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.
This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.
Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.
関連コンテンツ
Instructions