A0802
アジピン酸ジヒドラジド-アガロース
saline suspension
別名:
Adipic acid dihydrazide agarose beads
製品名
アジピン酸ジヒドラジド-アガロース, saline suspension
biological source
plant
form
saline suspension
extent of labeling
≥8 μmol per mL
technique(s)
microbe id | metabolite detection: suitable
matrix activation
cyanogen bromide
matrix spacer
11 atoms (when ligands (aldehydes, carboxylic acids) are coupled through free hydrazide groups)
suitability
suitable for chromatography
storage temp.
2-8°C
Quality Level
Physical form
Suspension in 0.5 M NaCl containing preservative.
Application
Adipic acid dihydrazide Agarose can be used in proteomics and protein chromatography. It has been utilized in research for purifying and identifying the fungal phytotoxin Fusicoccin (FC), identifying RNA binding proteins that interact with RNA cis-elements, and enzyme purification such as peroxisomes from guinea pig liver.
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
M Caputi et al.
The EMBO journal, 18(14), 4060-4067 (1999-07-16)
Splicing of the human immunodeficiency virus type 1 (HIV-1) pre-mRNA must be inefficient to provide a pool of unspliced messages which encode viral proteins and serve as genomes for new virions. Negative cis-regulatory elements (exonic splicing silencers or ESSs) are
S C Datta et al.
The Journal of biological chemistry, 265(14), 8268-8274 (1990-05-15)
The peroxisomal acyl/alkyl dihydroxyacetone-phosphate reductase (EC 1.1.1.101) was solubilized and purified 5500-fold from guinea pig liver. The enzyme could be solubilized by detergents only at high ionic strengths in presence of the cosubstrate NADPH. Peroxisomes, isolated from liver by a
Alice Tianbu Zhang et al.
Journal of cell science, 124(Pt 12), 2058-2069 (2011-05-26)
Non-coding Y RNAs are required for the initiation of chromosomal DNA replication in mammalian cells. It is unknown how they perform this function or if they associate with a nuclear structure during DNA replication. Here, we investigate the association of
Ruben Hovhannisyan et al.
BioTechniques, 46(2), 95-98 (2009-03-26)
Use of RNA affinity chromatography is commonly used to identify RNA binding proteins that interact with specific RNA cis-elements that function in post-transcriptional gene regulation. These purifications can be complicated by residual RNase activity in cellular extracts that can degrade
D Grate et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(11), 6131-6136 (1999-05-26)
The biological function of specific gene products often is determined experimentally by blocking their expression in an organism and observing the resulting phenotype. Chromophore-assisted laser inactivation using malachite green (MG)-tagged antibodies makes it possible to inactivate target proteins in a
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