A2064
抗ヒトIgG−アルカリフォスファターゼ抗体, マウスモノクロナール
clone GG-5, purified from hybridoma cell culture
別名:
モノクロナール抗ヒトIgG (γ-鎖特異的)
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この商品について
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
GG-5, monoclonal
Application:
direct ELISA
dot blot
immunohistochemistry
western blot (chemiluminescent)
dot blot
immunohistochemistry
western blot (chemiluminescent)
Species reactivity:
human
Citations:
13
Technique(s):
direct ELISA: 1:50,000
dot blot: 1:80,000
immunohistochemistry: suitable
western blot (chemiluminescent): 1:80,000
dot blot: 1:80,000
immunohistochemistry: suitable
western blot (chemiluminescent): 1:80,000
製品名
抗ヒトIgG−アルカリフォスファターゼ抗体, マウスモノクロナール, clone GG-5, purified from hybridoma cell culture
biological source
mouse
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
GG-5, monoclonal
form
buffered aqueous glycerol solution
species reactivity
human
technique(s)
direct ELISA: 1:50,000
dot blot: 1:80,000
immunohistochemistry: suitable
western blot (chemiluminescent): 1:80,000
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
液体沈降(Lppt)に適用。
Anti-Human IgG- Alkaline Phosphatase antibody, Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- western blotting
- dot blot
- immunohistology
Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).
General description
ヒトIgGと結合します。その他のヒトIg分子とは結合しません。抗体はヒトIgGのH鎖上の決定基に特異的です。本抗体は、3% PEG6000存在下の液層で抗原-抗体複合体を形成します。
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Monoclonal Anti-Human IgG (Γ-chain specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Physical form
0.05M Trisバッファ-溶液 (pH 8.0, 1mM MgCl2, 1%BSA, 50%グリセロ-ル, 15mMアジ化ナトリウム含有)。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 2
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A2064-1ML: + IXO97774: + A2064-BULK: + A2064-VAR: + A2064-.2ML:
jan
George J Broze
Blood cells, molecules & diseases, 52(2-3), 116-120 (2013-10-01)
Acquired factor X (FX) deficiency unrelated to amyloidosis is a rare disorder in which an anti-FX antibody is infrequently detected. A patient with severe bleeding due to a calcium ion-dependent anti-FX IgG antibody is described. The FX affinity purified IgG
Safety and Immunogenicity of a Pseudomonas aeruginosa Hybrid Outer Membrane Protein F-I Vaccine in Human Volunteers
Mansouri E, et al.
Infection and Immunity, 67(7), 1461-1470 (1999)
Werner E G Müller et al.
Journal of cell science, 128(11), 2202-2207 (2015-04-25)
Polyphosphate (polyP) is a physiologically occurring polyanion that is synthesized especially in bone-forming osteoblast cells and blood platelets. We used amorphous polyP nanoparticles, complexed with Ca(2+), that have a globular size of ∼100 nm. Because polyP comprises inorganic orthophosphate units
Receptor-mediated targeting of spray-dried lipid particles coformulated with immunoglobulin and loaded with a prototype vaccine
Bot AI, et al.
Pharmaceutical Research, 18(7), 971-979 (2001)
Camila A Wilkens et al.
PloS one, 10(3), e0119053-e0119053 (2015-03-15)
Cell engineering has been used to improve animal cells' central carbon metabolism. Due to the central carbon metabolism's inefficiency and limiting input of carbons into the TCA cycle, key reactions belonging to these pathways have been targeted to improve cultures'
関連コンテンツ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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