A2429
抗マウスIgG (Fc特異的)-アルカリフォスファターゼ ヤギ宿主抗体
affinity isolated antibody, buffered aqueous solution
別名:
Goat Anti-Mouse IgG (Fc specific)–AP
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この商品について
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
direct ELISA
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot (chemiluminescent)
Species reactivity:
mouse
Citations:
45
Technique(s):
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:160,000-1:320,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:160,000-1:320,000
製品名
抗マウスIgG (Fc特異的)-アルカリフォスファターゼ ヤギ宿主抗体, affinity isolated antibody, buffered aqueous solution
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse
should not react with
bovine, human, horse
technique(s)
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:160,000-1:320,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
PBS/0.1%Tweenおよび1%BSAで1：1000に希釈し、37°Cで1.5時間、ELISAアッセイでアルカリホスファターゼ結合ヤギ抗マウスFc特異抗体を二次抗体として使用しました。37°Cで30分間、基質として4-ニトロフェニルホスフェート（Sigma）を使用して抗体を開発しました。
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (1:1500) and western blot. The antibody can also be used for immunohistochemistry (1:40 using formalin-fixed, paraffin-embedded sections).
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA), sandwich ELISA and enzyme-linked immune absorbent spot (ELISPOT).
The product has been used as a secondary antibody for detecting mouse serum anti-hemagglutinin (HA) antibodies using ELISA techniques.
Other Notes
本抗体は、ウシ, ウマ, ヒト血清タンパク質で吸収済です。
Physical form
0.05M Tris溶液 (pH 8.0, 1%BSA, 1mM MgCl2 , 15mMアジ化ナトリウム含有)
Preparation Note
ウシ, ウマ, ヒトサンプルでのバックグラウンド染色の低減のため吸収処理済み。
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Immunogen
Purified mouse IgG, Fc fragment
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A2429-VAR: + A2429-.5ML: + A2429-.25ML: + A2429-1ML: + A2429-BULK:
jan
Gábor Oroszlán et al.
Frontiers in immunology, 8, 1821-1821 (2018-01-13)
Serine proteases (SPs) are typically synthesized as precursors, termed proenzymes or zymogens, and the fully active form is produced via limited proteolysis by another protease or by autoactivation. The lectin pathway of the complement system is initiated by mannose-binding lectin
Arnar Gudjonsson et al.
Journal of immunology (Baltimore, Md. : 1950), 198(7), 2785-2795 (2017-02-24)
Targeting Ags to conventional dendritic cells can enhance Ag-specific immune responses. Although most studies have focused on the induction of T cell responses, the mechanisms by which targeting improves Ab responses are poorly understood. In this study we present data
Marcel Zocher et al.
International immunology, 15(7), 789-796 (2003-06-17)
Antigen-specific B cells are key players in many autoimmune diseases through the production of autoreactive antibodies that can cause severe tissue damage and malfunction. We have designed and expressed a fusion protein, referred to as MOG-Fc, composed of the extracellular
The specificity of targeted vaccines for APC surface molecules influences the immune response phenotype
Grodeland G, et al.
Testing, 8(11), e80008-e80008 (2013)
Rathi Prasad et al.
The Journal of clinical investigation, 127(3), 942-953 (2017-02-07)
Primary adrenal insufficiency is life threatening and can present alone or in combination with other comorbidities. Here, we have described a primary adrenal insufficiency syndrome and steroid-resistant nephrotic syndrome caused by loss-of-function mutations in sphingosine-1-phosphate lyase (SGPL1). SGPL1 executes the
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Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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