A3688
抗マウスIgG (全分子)−アルカリフォスファターゼ ヤギ宿主抗体
affinity isolated antibody, buffered aqueous glycerol solution
別名:
Goat Anti-Mouse IgG (whole molecule)−AP
製品名
抗マウスIgG (全分子)−アルカリフォスファターゼ ヤギ宿主抗体, affinity isolated antibody, buffered aqueous glycerol solution
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
mouse
should not react with
human
technique(s)
direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
アルカリホスファターゼ抱合ヤギ抗-マウスIgGを、最終濃度0.5MのNaClを含むTBS/Tween中の濃度μg/mLで2次的に用いて、サーファクタントプロテインAが気管支肺胞液中に検出されました。
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody for immunocytochemistry assays and ELISA.
Human and Murine tumor cell lysate were analyzed for various protein expression by western blot using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary for 1 hour at 37 degrees.
Other Notes
本抗体は、ヒト血清タンパク質で吸収済です。
Physical form
0.05M Tris溶液 (pH 8.0, 1%BSA, 10mMグリシン, １mM MgCl2, 50%グリセロ-ル, 15mMアジ化ナトリウム含有)
Preparation Note
ヒトサンプルでのバックグラウンド低減のため吸収処理済み。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody react with normal mouse serum and mouse IgG using IEP. By ODD, the antiserum is reacts with mouse IgG1, IgG2a, IgG2b, IgG3, IgA and IgM. The conjugate shows no reactivity with human serum proteins by ELISA.
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody react with normal mouse serum and mouse IgG using IEP. By ODD, the antiserum is reacts with mouse IgG1, IgG2a, IgG2b, IgG3, IgA and IgM. The conjugate shows no reactivity with human serum proteins by ELISA.
Immunogen
purified mouse IgG
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保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Sandra Alejandra Serna-Salas et al.
BioMed research international, 2018, 4706976-4706976 (2019-01-16)
Regulation of the mechanisms of fibrosis is an important goal in the treatment of liver cirrhosis. One mechanism is the participation of hepatic stellate cells in fibrogenesis when activated by catecholamines. Consequently, α/β adrenoblockers are proposed as an alternative treatment
J P Donahue et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(25), 12178-12182 (1994-12-06)
We have developed a method for crystallizing small functional protein segments so that their three-dimensional structure can be determined by x-ray diffraction analysis. This method consists of linking a small protein segment of unknown tertiary structure to either the amino
Zahir Ali et al.
ACS synthetic biology, 11(1), 406-419 (2021-12-24)
Simple, rapid, specific, and sensitive point-of-care detection methods are needed to contain the spread of SARS-CoV-2. CRISPR/Cas9-based lateral flow assays are emerging as a powerful alternative for COVID-19 diagnostics. Here, we developed Bio-SCAN (biotin-coupled specific CRISPR-based assay for nucleic acid
Peter Traxler et al.
Cancer research, 64(14), 4931-4941 (2004-07-17)
Aberrant epidermal growth factor receptor (EGFR) and ErbB2 expression are associated with advanced disease and poor patient prognosis in many tumor types (breast, lung, ovarian, prostate, glioma, gastric, and squamous carcinoma of head and neck). In addition, a constitutively active
Catharina E Dam et al.
Scandinavian journal of clinical and laboratory investigation, 74(6), 506-514 (2014-05-06)
Knowledge about antibody-antigen interactions is important for the understanding of the immune system mechanisms and for supporting development of drugs and biomarkers. A tool for identification of these antigenic epitopes of specific antibodies is epitope mapping. In this study, a
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Instructions
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