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Merck

A4312

抗マウスIgG (全分子)−アルカリフォスファターゼ ウサギ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

別名:

Rabbit Anti-Mouse IgG (whole molecule)−AP

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162640
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
52

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biological source

rabbit

Quality Level

300

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

mouse

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000 using using β-Actin in total cell extract of HeLa cells (5-10 μg/well)

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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関連するカテゴリー

General description

IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid, capable of fighting bacterial and viral infection.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Immunogen

Purified mouse IgG.

Application

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in immunocytochemical localization of Aβ peptides and western blot analysis.
Anti-mouse IgG (whole molecule)-alkaline phosphatase antibody can be used in immunocytochemistry.
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

0.05 M Trisバッファ-溶液 (pH 8.0, 1mM MgCl2, 10mM グリシン, 1%BSA, 50%グリセロ-ル, 15mMアジ化ナトリウム含有)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

A4312-1ML: + A4312-VAR: + A4312-.5ML: + A4312-BULK: + A4312-.25ML: + A4312-.05ML:

jan

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試験成績書（COA）

Lot/Batch Number
0000349957
0000373536
0000467684
0000524211
0000534828

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文書ライブラリにアクセスする

Cesar Andres Diaz Arias et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 48(3), 419-426 (2017-02-27)
Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain
E Przygrodzka et al.
Reproduction (Cambridge, England), 151(2), 135-147 (2015-11-19)
In order to characterize the transition of the corpora lutea (CL) from acquisition of luteolytic sensitivity to rescue of luteal function: i) the expression of 38 factors associated with steroids, prostanoids, and angiogenic systems and ii) concentrations of the main
Fernando M Diaz et al.
Virology, 488, 61-67 (2015-11-27)
Viral vaccines have traditionally protected against disease, but for viruses that establish latent infection, it is desirable for the vaccine to reduce infection to reduce latent infection and reactivation. While seroconversion has been used in clinical trials of herpes simplex
beta APP and furin mRNA concentrates in immature senile plaques in the brain of Alzheimer patients
Marcinkiewicz M
Journal of Neuropathology and Experimental Neurology, 61(9), 815-829 (2002)
Sarah Schulz et al.
EMBO reports, 18(4), 526-535 (2017-03-12)
The genome of the highly infectious bacterium
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