A4464
Enhanced Avian逆転写酵素 [eAMV™RT]
For reverse transcription at higher temperatures & rare mRNAs
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この商品について
Concentration:
20 units/μL
Quality Level
form
crystalline powder
usage
sufficient for 50 reactions
feature
dNTPs included: no, hotstart: no
concentration
20 units/μL
technique(s)
RT-PCR: suitable, cDNA synthesis: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
General description
eAMV™ Reverse Transcriptase is an enhanced form of Avian Myeloblastosis Virus (AMV) RT that synthesizes a DNA strand complementary to RNA, DNA, or an RNA:DNA hybrid. This exceptionally robust AMV RT has greater thermostability than standard AMV or Moloney murine leukemia virus(M-MLV) reverse transcriptase. eAMV™ RT is an ideal enzyme for producing high-quality full-length cDNA from total RNA or poly(A)+ RNA and is also efficient at transcribing long targets.
Application
Enhanced Avian (eAMV) Reverse Transcriptase is a highly purified, avian myeloblastosis virus reverse transcriptase (AMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT).
This exceptionally robust AMV-RT has an enhanced ability to detect low abundance messages, transcribe through difficult secondary structure at elevated temperatures (up to 65 °C), and transcribe mRNA templates up to 14.1 kb.
This exceptionally robust AMV-RT has an enhanced ability to detect low abundance messages, transcribe through difficult secondary structure at elevated temperatures (up to 65 °C), and transcribe mRNA templates up to 14.1 kb.
Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.
Enhanced Avian Reverse Transcriptase [eAMV™ RT] has been used for reverse transcription of total RNA to synthesize cDNA during quantitative reverse transcription PCR (RT-qPCR) analysis.
Biochem/physiol Actions
Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.
Features and Benefits
- Greater sensitivity for low abundance mRNA
- Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
- Efficient generation of full-length cDNA, up to 14.1 kb
- Produces first strand cDNA ready for PCR amplification
Packaging
Provided with a vial of 10× reaction buffer.
Other Notes
One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)12-18 as a primer.
Legal Information
本製品の購入は研究のみを目的とし、サ-マルサイクラ-を使ってポリメラ-ゼ連鎖反応(PCR)処理を実施する場合に限ってその使用が認められます。自動化PCR処理に伴うサ-マルサイクラ-の利用は、Applied Biosystems社への支払いまたは正規に認められたサ-マルサイクラ-の購入による、ライセンス料の前払いによってカバ-されます。
eAMV is a trademark of Sigma-Aldrich Co. LLC
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
資料
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
遺伝子発現分析における課題には、mRNA安定性、時間的転写パターン、mRNAとタンパク質の相関などがあり、正確さに影響を及ぼしています。
関連コンテンツ
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
RT-qPCRは、遺伝子発現や病原体検出における用途で、特定のターゲットを検出します。
Instructions
Emily Schifano et al.
Virulence, 10(1), 1013-1025 (2019-11-28)
Calcium signaling can elicit different pathways involved in an extreme variety of biological processes. Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology. The Ca2+-ATPase
Y M Zhu et al.
British journal of cancer, 91(11), 1970-1976 (2004-11-17)
Interleukin-8/CXCL8 (IL-8) is a chemokine and angiogenic factor. Recently, IL-8 was identified as an autocrine growth factor in several human cancers. Here, we investigated the expression and function of IL-8 in lung cancer cells. The expressions of IL-8 and its
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 5-5 (1989)