モノクローナル抗VSV-G抗体-ペルオキシダーゼ標識 マウス宿主抗体

VSV糖タンパク質のC末端の5アミノ酸を含有するエピト-プを認識します。感染細胞において、細胞表面・出芽ウイルスに存在する成熟型VSV-Gおよび、粗面小胞体(RER)・ゴルジ複合体偏平嚢に存在する未成熟型VSV-Gも検出します。膜および細胞質ドメインを欠損したVSV-Gの分泌型とは反応しません。新規に合成されたVSV-Gが原形質膜・細胞表面に移動する間の細胞質ドメインの働きに関する研究における、マイクロインジェクション, イムノブロッティング, 免疫沈降法, 免疫細胞化学, 免疫電子顕微鏡法などに本抗体が用いられました。本抗体は、細胞外に発現するVSV-GのC末端でタグ標識した構造の検出, 免疫沈降, 免疫細胞化学的染色するのに用いられます。このタグは目的タンパク質の機能に干渉しません。また、各種の内因性タンパク質と交差反応のない、特異的なP5D4抗体で検出できます。

融合タンパク質のN末端またはC末端に発現した時の、VSV-Gタグ融合タンパク質上のVSV-Gタグ配列(YTDIEMNRLGK)。天然型および変性型のVSV-Gタグタンパク質を認識します。

Vesicular Stomatitis Virus Glycoprotein (VSV-G) amino acid sequence YTDIEMNRLGK, corresponding to amino acids 501-511 has been widely used as an epitope tag in expression vectors, enabling the expression of proteins as VSV-G tagged fusion proteins. It constitutes an attractive model to study maturation and intracellular transport of membrane proteins. VSV-G mediates attachment of VSV to the cell surface and induces pH-dependent fusion between viral and target membranes. Besides, its cytoplasmic domain contains information for several intracellular sorting steps, which include efficient export from the endoplasmic reticulum, basolateral delivery and endocytosis..VSV-G is transported through stacks in the Golgi cisternae.

KLHと結合した、水疱性口内炎ウイルス糖タンパク質(VSV-G)のC末端アミノ酸15残基(497~511)を含有する合成ペプチドのKLH結合体。

Monoclonal Anti-VSV-G−Peroxidase antibody produced in mouse may be used immunoblotting.

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Monoclonal Anti-VSV-G-Peroxidase is a lyophilized preparation of the purified immunoglobulin fraction of monoclonal Anti-VSV-G (mouse IgG1 isotype) isolated from ascites fluid of the P5D4 hybridoma, conjugated to horseradish peroxidase (HRP). The antibody is derived from the P5D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide. Vesicular Stomatitis Virus Glycoprotein (VSV-G) exists as a trimer and comprises of four domains, among which domain III is a pleckstrin homology (PH) domain.

Supplied as a lyophilized powder. After reconstitution,the solution contains 1% BSA and 0.05% MIT in 0.01 M sodium phosphate buffered saline, pH 7.4.

Reconstitution with 0.5 mL water results in a solution of 0.01 M sodium phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% MIT.

Stored the lyophilized product at 2-8 °C. After reconstitution, for extended storage, freeze in working aliquots at –20 °C. For continuous use, the solution may be store at 2-8 °C for up to 1 month. Working dilutions should be discarded. Avoid repeated freeze-thaw.