A9226
アルカリ性バッファー 溶液
1.5 M, pH 10.3 (25 °C)
別名:
ベーシック pH バッファー
Application
アルカリバッファー溶液は、以下の物質中のアルカリホスファターゼ（ALP）活性の測定に使用されています：
- ラット骨髄間質細胞（BMSC）
- 骨芽細胞様細胞（SaoS-2、ATCC HTB 85）
- マウスMC3T3-E1
General description
アルカリホスファターゼ反応には、アルカリバッファー溶液（1.5M、pH 10.3、25℃）を使用します。2-アミノ-2-メチルプロパノール（AMP）を含有し、p-ニトロフェニルリン酸（pNPP）酵素基質とともに使用されます。
Other Notes
2-アミノ-2-メチルプロパノール含有
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Young-Hee Kim et al.
BMC pharmacology & toxicology, 14, 60-60 (2013-12-03)
Indoxyl sulfate (IS), an organic anion uremic toxin, promotes the progression of renal dysfunction. Some studies have suggested that IS inhibits osteoclast differentiation and suppresses parathyroid hormone (PTH)-stimulated intracellular cAMP production, decreases PTH receptor expression, and induces oxidative stress in
Pin Huang et al.
Colloids and surfaces. B, Biointerfaces, 160, 628-638 (2017-10-17)
Increased use of reconstruction procedures in orthopedics has improved the life of patients undergoing surgery. However, surgical site infection remains a major challenge. Efforts were made to fabricate antibacterial surfaces with good biocompatibility. This present study aimed to fabricate zinc-incorporated
Salima Nedjari et al.
Scientific reports, 7(1), 15947-15947 (2017-11-23)
Stem cells therapy offers a viable alternative for treatment of bone disorders to the conventional bone grafting. However clinical therapies are still hindered by the insufficient knowledge on the conditions that maximize stem cells differentiation. Hereby, we introduce a novel
C Caparrós et al.
Journal of the mechanical behavior of biomedical materials, 39, 79-86 (2014-08-12)
The generation of titanium foams is a promising strategy for modifying the mechanical properties of intervertebral reinforcements. Thus, the aim of this study was to compare the in vitro biological response of Ti6Al4V alloys with different pore sizes for use
Helle Lysdahl et al.
BioResearch open access, 3(6), 278-285 (2014-12-04)
Clinical trials using bone morphogenetic protein-2 (BMP2) for bone reconstruction have shown promising results. However, the relatively high concentration needed to be effective raises concerns for efficacy and safety. The aim of this study was to investigate the osteogenic effect
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Instructions
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