A9316
抗マウスIgG (全分子)−アルカリフォスファターゼ ヤギ宿主抗体
affinity isolated antibody, buffered aqueous glycerol solution
別名:
Goat Anti-Mouse IgG (whole molecule)−AP
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この商品について
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
31
biological source
goat
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
technique(s)
direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.
Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Application
アルカリホスファターゼ抱合ヤギ抗-マウスIgGを、最終濃度0.5MのNaClを含むTBS/Tween中の濃度μg/mLで2次的に用いて、サーファクタントプロテインAが気管支肺胞液中に検出されました。
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:
- histological studies in heart and liver grafts
- immunohistochemical studies of gangliosides and immunostaining of lymphoid organs
- western blot detection in blood samples
The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.
Biochem/physiol Actions
IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.
Physical form
0.05 M Tris溶液(pH 8.0, 10 mMグリシン, 1% BSA, 1 mM MgCl2, 50%グリセロ-ル, 15 mMアジ化ナトリウム含有)。
Preparation Note
バックグラウンド低減のため、ラットサンプルで吸収済みです。
Other Notes
ラット血清タンパクで吸収済みの抗体です。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A9316-BULK: + A9316-.25ML: + A9316-VAR: + A9316-1ML-KC: + A9316-1ML: + A9316-.5ML: + A9316PROC:
jan
Alcohol administration blocks stress-induced impairments in memory and anxiety, and alters hippocampal neurotransmitter receptor expression in male rats
Gomez JL, et al.
Hormones and Behavior, 63(4), 659-666 (2013)
Prolonged Cardiac Allograft Survival In Rats Systemically Injected Adenoviral Vectors Containing Ctla4ig-gene1
Kita Y, et al.
Transplantation, 68(6), 758-766 (1999)
Significant enhancement by anti-ICOS antibody of suboptimal tacrolimus immunosuppression in rat liver transplantation
Guo L, et al.
Liver Transplantation, 10(6), 743-747 (2004)
Simultaneous immunohistochemical detection of gangliosides and neuronal markers in paraformaldehyde-fixed nervous tissues by acetone etching
Sakumoto Y, et al.
Archives of Histology and Cytology, 72(2), 77-90 (2009)
The microstructure of secondary lymphoid organs that support immune cell trafficking
Matsuno K, et al.
Archives of Histology and Cytology, 73(1), 1-21 (2010)
関連コンテンツ
Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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