B2025
ブリリアントブルーG - コロイド濃縮液
関連するカテゴリー
General description
Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.
Application
Brilliant Blue G - Colloidal Concentrate has been used in the staining
- protein fragments post in vitro pepsin digestion
- ovarian tumor fluid proteome prior to profiling
- the transcription factor (TFIID) complex from Saccharomyces cerevisiae
Preparation Note
水800 mLで1リットルに希釈すると、懸濁液は0.1%(w/v)Brilliant Blue G, 0.29Mリン酸, 16%飽和硫酸アンモニウム含有となります。
Analysis Note
SDS-PAGE適合試験済みです。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
保管分類
8B - Non-combustible corrosive hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
名称等を表示すべき危険物及び有害物
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名称等を通知すべき危険物及び有害物
ishl_notified
B2025-1EA:4548174007365 + B2025-VAR: + B2025-BULK: + B2025PROC:
jan
Julie Ducreux et al.
Bioconjugate chemistry, 19(10), 2088-2094 (2008-09-24)
PEGylation of antibodies is known to increase their half-life in systemic circulation, but nothing is known regarding whether PEGylation can improve the inhibitory potency of antibodies against target receptors. In this paper, we have examined this question using antibodies directed
Roy Auty et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004-09-28)
The basal transcription factor TFIID is composed of the TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Although TBP alone binds to the TATA box of DNA and supports basal transcription, the TAFs have essential functions that remain poorly defined.
Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
Dyballa N and Metzger S
Journal of Visualized Experiments (2009)
Purification of Active TFIID from Saccharomyces cerevisiae EXTENSIVE PROMOTER CONTACTS AND CO-ACTIVATOR FUNCTION
Auty R, et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004)
A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins
Thomas K, et al.
Regulatory Toxicology and Pharmacology, 39(2), 87-98 (2004)
プロトコル
Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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