F0427
Fasリガンド ヒト
>95% (SDS-PAGE), recombinant, expressed in CHO cells, lyophilized powder
別名:
FasL
製品名
Fasリガンド ヒト, >95% (SDS-PAGE), recombinant, expressed in CHO cells, lyophilized powder
recombinant
expressed in CHO cells
assay
>95% (SDS-PAGE)
form
lyophilized powder
mol wt
monomer calculated mol wt ~18 kDa
26-28 kDa by SDS-PAGE
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... FASLG(356)
Analysis Note
活性は、ジャ-カット細胞のアポト-シス誘導能により測定しています。
Biochem/physiol Actions
Fasリガンドは腫瘍壊死因子(TNF)ファミリ-のサイトカインに属するタンパク質であり、 細胞膜レセプタ-Fas(CD95/Apo-1)を発現する細胞のアポト-シスを引き起こします。
腫瘍壊死因子(TNF)ファミリ-のサイトカインに属するタンパク質であり、 細胞膜レセプタ-Fas (CD95/Apo-1)を発現する細胞のアポト-シスを引き起こします。
FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E. In CD4+ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.
Other Notes
N末端6Xヒスチジンタグ付加ヒトFasリガンドは、Fasのアミノ酸134-281をコ-ドしています。
Physical form
凍結乾燥品 (PBS溶液, 0.5mg BSA含有, 0.2μmフィルタ-滅菌済み)。
Application
Fas Ligand (FASLG) human has been used for-
- the study of FASLG stimulation in luteal cells obtained from mid-corpus luteum (CL) and
- the study of apoptosis induction and intracellular caspase-3 assay in human cells.
General description
FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.
signalword
Warning
hcodes
pcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
F0427-10UG: + F0427-VAR: + F0427-BULK: + F0427-10UG-PW:
jan
Aleksander Szymanowski et al.
Atherosclerosis, 233(2), 616-622 (2014-02-19)
Apoptosis of natural killer (NK) cells is increased in patients with coronary artery disease (CAD) and may explain why NK cell levels are altered in these patients. Soluble forms of Fas and Fas ligand (L) are considered as markers of
Xiaoting Jin et al.
PloS one, 9(12), e113257-e113257 (2014-12-03)
Dichlorodiphenoxytrichloroethane (DDT) is a known persistent organic pollutant and liver damage toxicant. However, there has been little emphasis on the mechanism underlying liver damage toxicity of DDT and the relevant effective inhibitors. Hence, the present study was conducted to explore
Malavika S Giri et al.
Journal of immunology (Baltimore, Md. : 1950), 182(7), 4459-4470 (2009-03-21)
Mechanisms that may allow circulating monocytes to persist as CD4 T cells diminish in HIV-1 infection have not been investigated. We have characterized steady-state gene expression signatures in circulating monocytes from HIV-infected subjects and have identified a stable antiapoptosis gene
Antonio M Galvao et al.
Biology of reproduction, 83(6), 901-908 (2010-08-20)
Proapoptotic factor Fas ligand (FASL) and its cell surface receptor FAS are tumor necrosis factor superfamily members that trigger apoptosis in different cell types. However, their influence on luteal steroidogenesis is not clearly understood. The aim of the present work
Intracellular bacteria engage a STING-TBK1-MVB12b pathway to enable paracrine cGAS-STING signalling.
Ramya Nandakumar et al.
Nature microbiology, 4(4), 701-713 (2019-02-26)
The innate immune system is crucial for eventual control of infections, but may also contribute to pathology. Listeria monocytogenes is an intracellular Gram-positive bacteria and a major cause of food-borne disease. However, important knowledge on the interactions between L. monocytogenes
関連コンテンツ
Datasheet
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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