L3272
ルリアアガーベース(ミラー改変)
Powder microbial growth medium
別名:
Luria Agar Base (Miller’s Modification)
grade
Molecular Biology
sterility
non-sterile
form
powder
composition
Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(3.05% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
Quality Level
関連するカテゴリー
General description
Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.
Application
LB Broth with agar (Luria low salt) has been used:
- for culturing E. coli DH10B cells.
- in the isolation of diazotrophic bacteria.
- for transformation of competent E. coli strain Stbl3 for cloning.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Features and Benefits
Luria′s low salt LB powder with agar provides:
- Easy scale-up using larger package sizes
- A budget-friendly alternative to pre-poured plates
- Standard formulation
Preparation Note
1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.
Legal Information
Zeocin is a registered trademark of Cayla Sarl
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
L3272-BULK: + L3272-VAR: + L3272-1KG-PW: + L3272-1KG: + L3272-250G:
jan
Increased Expression of a Reporter Plasmid by Crotalid Venom Fraction
Islas AA, et al.
Journal of Animal and Veterinary Advances, 9(17), 2214-2219 (2010)
Using plant growth-promoting rhizobacteria (PGPR) to improve plant growth
Grobelak A, et al.
Ecological Engineering, 84, 22-28 (2015)
Difco Manual, 271-271 (1998)
Vidya Padmanabhan Nair et al.
STAR protocols, 3(2), 101281-101281 (2022-04-26)
Human endogenous retroviruses (HERVs) comprise many regulatory elements and can regulate host gene activity at different expression levels via multiple mechanisms. Here, we introduce a step-by-step protocol to activate or repress transcription of HERV-K(HML-2) elements using the CRISPRa and CRISPRi
Miller, J.H.
Experiments in Molecular Genetics, 433-433 (1972)
プロトコル
General protocols for growth of competent cells in microbial medium.
関連コンテンツ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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