S5394
リポタンパク質欠損血清 ウシ胎仔由来
frozen liquid
別名:
ウシ胎児血清, リポタンパク質欠損血清
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この商品について
NACRES:
NA.61
UNSPSC Code:
12352207
Biological source:
bovine fetus
Form:
frozen liquid
Shipped in:
dry ice
biological source
bovine fetus
Quality Level
form
frozen liquid
concentration
≥14 mg protein/mL Biuret
impurities
<1 ng/mg protein endotoxin, <5 % of normal lipoprotein
shipped in
dry ice
storage temp.
−20°C
General description
ヒト線維芽細胞をリポタンパク質が欠乏した血清中で培養すると、ステロールの生成が低下することが、いくつかの試験で明らかにされています。
Application
ウシ胎児由来のリポタンパク質除去血清は、チャイニーズハムスター卵巣（CHO）細胞、胸腺小葉およびヒト末梢血単核細胞（PBMC）の成長培地のサプリメントとして使用されています。
Preparation Note
0.2 μmフィルタ-で無菌濾過済
ダルベッコのリン酸緩衝食塩水に対して透析、pH 7.4～7.5
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)
P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Ying Wang et al.
Cell death discovery, 7(1), 201-201 (2021-08-06)
Coenzyme Q (CoQ) is a lipid-like mobile electron transporter of the mitochondrial respiratory chain. Patients with partial loss-of-function mutations in the CoQ biosynthesis pathway suffer from partial primary CoQ deficiency (MIM 607426). This leads to mitochondrial dysfunction, which presents like
プロトコル
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
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