TOX6
In Vitro毒性アッセイキット(スルホローダミンBベース)
別名:
biomass viability assay, sulforhodamine B, total biomass assay
form
liquid
usage
kit sufficient for 1,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
565 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
room temp
Quality Level
Application
In Vitro毒性アッセイキット（スルホローダミンBベース）は、総タンパク質によるバイオマス（生細胞および非生細胞）の分光光度測定に使用されています。また、ぶどう膜黒色腫細胞5株の増殖に対する17-アリルアミノ-17-デメトキシゲルダナマイシン（17-AAG）の作用を研究するために使用されています。波長565 nmにおける色素の吸光度を測定します。
Biochem/physiol Actions
色素は細胞タンパク質に結合し、塩基で可溶化されます。
General description
スルホローダミンB法は簡便かつ正確で、再現性のある結果が得られます。このアッセイ系は、細胞タンパク質をスルホローダミンBで染色することにより総バイオマスを測定する手法です。
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A
保管分類
3 - Flammable liquids
P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Marike Gabrielson et al.
PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated
Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
Santos F, eta l.
Endocrine-related cancer, 21(1), 1-40 (2014)
Sofia Oliveira-Pinto et al.
Bioorganic chemistry, 100, 103942-103942 (2020-05-26)
A selection of new chromeno[2,3-b]pyridines was prepared from chromenylacrylonitriles and N-substituted piperazines, using a novel and efficient synthetic procedure. The compounds were tested for their anticancer activity using breast cancer cell lines MCF-7, Hs578t and MDA-MB-231 and the non-neoplastic cell
Evaluation of colorimetric protein and biomass stains for assaying drug effects upon human tumor cell lines.
Skehan, P., et al.
Proceedings of the American Association For Cancer Research, 30, 2436-2436 (1989)
資料
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
関連コンテンツ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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