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AB1924 Anti-Integrin α4 Antibody, cytoplasmic on CT

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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, Ht, M, Po, RELISA, IP, RIA, WB, IHCRbSerumPolyclonal Antibody
      Catalogue NumberAB1924
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin α4 Antibody, cytoplasmic on CT
      Alternate Names
      • CD49d
      Product Information
      PresentationLiquid rabbit antiserum with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-Integrin α4 Antibody, cytoplasmic on C-terminus is an antibody against Integrin α4 for use in ELISA, IP, RIA, WB, IH.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Radioimmunoassay
      • Western Blotting
      • Immunohistochemistry
      Application NotesELISA/RIA: 1:1000.

      Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.

      Immunohistochemistry: 1:500

      Immunoprecipitation: 5 μL of antibody is sufficient to precipitate alpha4 from 5x106 cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha 4 integrin subunit.
      Epitopecytoplasmic domain, C-terminus
      SpecificityIntegrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular.
      Species Reactivity
      • Chicken
      • Human
      • Hamster
      • Mouse
      • Pig
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain.
      Gene Symbol
      • ITGA4
      • MGC90518
      • VLA-4
      • IA4
      • CD49D
      • CD49d
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13612 # Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
      SIZE: 1038 amino acids; 115333 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P13612 The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
      PTM: Phosphorylation on Ser-1027 inhibits PXN binding.
      SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20ºC in undiluted aliquots for up to 12 months upon date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Número de referencia GTIN
      AB1924 04053252737886


      Anti-Integrin α4 Antibody, cytoplasmic on CT Certificados de análisis

      CargoNúmero de lote
      RABBIT ANTI-HUMAN INTEGRIN alpha4 - 2953892 2953892
      RABBIT ANTI-HUMAN INTEGRIN alpha4 - 3583832 3583832
      RABBIT ANTI-HUMAN INTEGRIN alpha4 - 3888748 3888748
      RABBIT ANTI-HUMAN INTEGRIN alpha4 - 4073059 4073059
      RABBIT ANTI-HUMAN INTEGRIN alpha4 -2681220 2681220
      RABBIT ANTI-HUMAN INTEGRIN alpha4 -2751186 2751186
      RABBIT ANTI-HUMAN INTEGRIN alpha4 -2814679 2814679

      Referencias bibliográficas

      Visión general referenciasAplicación EspeciePub Med ID
      Quiescence and activation of stem and precursor cell populations in the subependymal zone of the mammalian brain are associated with distinct cellular and extracellular matrix signals.
      Kazanis, Ilias, et al.
      J. Neurosci., 30: 9771-81 (2010)  2009

      Mostrar resumen
      20660259 20660259
      Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in apoE-deficient mice.
      Wang, F; Okamoto, Y; Inoki, I; Yoshioka, K; Du, W; Qi, X; Takuwa, N; Gonda, K; Yamamoto, Y; Ohkawa, R; Nishiuchi, T; Sugimoto, N; Yatomi, Y; Mitsumori, K; Asano, M; Kinoshita, M; Takuwa, Y
      The Journal of clinical investigation  120  3979-95  2009

      Mostrar resumen
      20978351 20978351
      Enhanced focal adhesion assembly reflects increased mechanosensation and mechanotransduction at maternal-conceptus interface and uterine wall during ovine pregnancy.
      Burghardt, RC; Burghardt, JR; Taylor, JD; Reeder, AT; Nguen, BT; Spencer, TE; Bayless, KJ; Johnson, GA
      Reproduction (Cambridge, England)  137  567-82  2009

      Mostrar resumen
      Immunofluorescence19060096 19060096
      Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration.
      Erikson, DW; Burghardt, RC; Bayless, KJ; Johnson, GA
      Biology of reproduction  81  814-25  2009

      Mostrar resumen
      19571258 19571258
      Integrins mediate adherence and migration of T lymphocytes on human peritoneal mesothelial cells.
      Wang, HH; Lee, TY; Lin, CY
      Kidney international  74  808-16  2008

      Mostrar resumen
      18614994 18614994
      Integrin expression and osteopontin regulation in human fetal osteoblastic cells mediated by substratum surface characteristics.
      Jung Yul Lim, Amanda F Taylor, Zhongyong Li, Erwin A Vogler, Henry J Donahue
      Tissue engineering  11  19-29  2004

      Mostrar resumen
      15738658 15738658
      Expression of alphav, alpha4, alpha5 and beta3 integrin subunits, fibronectin and vitronectin in goat peri-implantation
      Garcia, P. et al.
      Anim. Reprod. Sci., 80(1-2):91-100 (2004)  2004

      15036518 15036518
      Squamous cell carcinoma cell aggregates escape suspension-induced, p53-mediated anoikis: fibronectin and integrin alphav mediate survival signals through focal adhesion kinase.
      Zhang, Y; Lu, H; Dazin, P; Kapila, Y
      The Journal of biological chemistry  279  48342-9  2004

      Mostrar resumen
      Western BlottingHuman15331608 15331608
      Active detachment involves inhibition of cell-matrix contacts of malignant melanoma cells by secretion of melanoma inhibitory activity.
      Anja-Katrin Bosserhoff, Raphael Stoll, Jonathan P Sleeman, Frauke Bataille, Reinhard Buettner, Tad A Holak
      Laboratory investigation; a journal of technical methods and pathology  83  1583-94  2003

      Mostrar resumen
      14615412 14615412
      Regulation of integrin function by CD47 ligands. Differential effects on alpha vbeta 3 and alpha 4beta1 integrin-mediated adhesion.
      Barazi, HO; Li, Z; Cashel, JA; Krutzsch, HC; Annis, DS; Mosher, DF; Roberts, DD
      The Journal of biological chemistry  277  42859-66  2002

      Mostrar resumen
      12218055 12218055

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