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17-321 Rap1 Activation Assay Kit

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17-321
1 kit  Kit capacity: 20-30 assays
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      Descripción

      Replacement Information

      Tabla espec. clave

      Key Applications
      ABA
      Description
      Catalogue Number17-321
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionRap1 Activation Assay Kit
      References
      Product Information
      Components
      • 100X GTPγS, 10mM (Cat.# 20-176)
      • 100X GDP, 100mM (Cat.# 20-177)
      • Rap1 Activation Lysis Buffer, 2X
      • Anti-Rap1
      • Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
      HS Code3002 15 90
      Quality LevelMQ100
      Applications
      ApplicationNon-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.
      Key Applications
      • Affinity Binding Assay
      Biological Information
      Entrez Gene Number
      Gene Symbol
      • RAP1GDS1
      • MGC118859
      • GDS1
      • RAP1
      • MGC118861
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P52306 # Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein.
      SIZE: 607 amino acids; 66403 Da
      SIMILARITY: SwissProt: P52306 ## Contains 5 ARM repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 20-30 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      17-321 04053252369438

      Documentation

      Rap1 Activation Assay Kit Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Rap1 Activation Assay Kit Certificados de análisis

      CargoNúmero de lote
      Rap1 Activation Assay Kit 3011042
      Rap1 Activation Assay Kit 2886123
      Rap1 Activation Assay Kit 2918797
      Rap1 Activation Assay Kit 2844014
      Rap1 Activation Assay Kit 3113359
      Rap1 Activation Assay Kit 3117889
      Rap1 Activation Assay Kit - 2089003 2089003
      Rap1 Activation Assay Kit - 2140555 2140555
      Rap1 Activation Assay Kit - 2359951 2359951
      Rap1 Activation Assay Kit - 2388888 2388888

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Measurement of GTP-bound Ras-like GTPases by activation-specific probes.
      van Triest, M, et al.
      Meth. Enzymol., 333: 343-8 (2001)  2001

      11400350 11400350
      Signaling of hepatocyte growth factor/scatter factor (HGF) to the small GTPase Rap1 via the large docking protein Gab1 and the adapter protein CRKL.
      Sakkab, D, et al.
      J. Biol. Chem., 275: 10772-8 (2000)  1999

      Mostrar resumen
      10753869 10753869
      Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction
      Rebhun, J F, et al
      J Biol Chem, 275:34901-8 (2000)  1999

      10934204 10934204
      Rapid Ca2+-mediated activation of Rap1 in human platelets.
      Franke, B, et al.
      EMBO J., 16: 252-9 (1997)  1997

      Mostrar resumen
      9029146 9029146
      Differential interaction of the ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor
      Herrmann, C, et al
      J Biol Chem, 271:6794-800 (1996)  1996

      8636102 8636102

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