17-321 Rap1 Activation Assay Kit

17-321
1 kit  Kit capacity: 20-30 assays
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Key Applications
      ABA
      Description
      Catalogue Number17-321
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionRap1 Activation Assay Kit
      References
      Product Information
      Components
      • 100X GTPγS, 10mM (Cat.# 20-176)
      • 100X GDP, 100mM (Cat.# 20-177)
      • Rap1 Activation Lysis Buffer, 2X
      • Anti-Rap1
      • Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
      Applications
      ApplicationNon-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.
      Key Applications
      • Affinity Binding Assay
      Biological Information
      Entrez Gene Number
      Gene Symbol
      • RAP1GDS1
      • MGC118859
      • GDS1
      • RAP1
      • MGC118861
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P52306 # Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein.
      SIZE: 607 amino acids; 66403 Da
      SIMILARITY: SwissProt: P52306 ## Contains 5 ARM repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 20-30 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Rap1 Activation Assay Kit FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Rap1 Activation Assay Kit Certificats d'analyse

      TitreNuméro de lot
      Rap1 Activation Assay Kit2918797
      Rap1 Activation Assay Kit3011042
      Rap1 Activation Assay Kit2844014
      Rap1 Activation Assay Kit2886123
      Rap1 Activation Assay Kit3113359
      Rap1 Activation Assay Kit3117889
      Rap1 Activation Assay Kit - 20890032089003
      Rap1 Activation Assay Kit - 21405552140555
      Rap1 Activation Assay Kit - 23599512359951
      Rap1 Activation Assay Kit - 23888882388888

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Measurement of GTP-bound Ras-like GTPases by activation-specific probes.
      van Triest, M, et al.
      Meth. Enzymol., 333: 343-8 (2001)  2001

      11400350 11400350
      Signaling of hepatocyte growth factor/scatter factor (HGF) to the small GTPase Rap1 via the large docking protein Gab1 and the adapter protein CRKL.
      Sakkab, D, et al.
      J. Biol. Chem., 275: 10772-8 (2000)  2000

      Afficher le résumé
      10753869 10753869
      Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction
      Rebhun, J F, et al
      J Biol Chem, 275:34901-8 (2000)  2000

      10934204 10934204
      Rapid Ca2+-mediated activation of Rap1 in human platelets.
      Franke, B, et al.
      EMBO J., 16: 252-9 (1997)  1997

      Afficher le résumé
      9029146 9029146
      Differential interaction of the ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor
      Herrmann, C, et al
      J Biol Chem, 271:6794-800 (1996)  1996

      8636102 8636102

      Brochure

      Titre
      Hallmarks of Aging

      Produits & Applications associés

      Kit Component

      Référence Description  
      14-455 Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg Prix & Disponibilité
      20-176 100X GTPγS, 10mM Prix & Disponibilité
      20-177 100X GDP, 100mM Prix & Disponibilité

      Catégories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays