115943 | Methylene blue (C.I.52015)

115943
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      Overview

      Replacement Information

      Key Spec Table

      EC NumberHill FormulaChemical FormulaMolar Mass
      200-515-2C₁₆H₁₈ClN₃S * x H₂OC₁₆H₁₈ClN₃S * x H₂O (x=2-3)319.86 g/mol (anhydrous)

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      1159430025
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          Glass bottle 25 g
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          1159430100
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              Glass bottle 100 g
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              Description
              Catalogue Number115943
              SynonymsMethylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
              DescriptionMETHYLENE BLUE (C.I. 52015) FOR MICROSCO
              OverviewMethylene blue (C.I. 52015) is a Certistain® dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration.
              References
              Product Information
              EC number200-515-2
              Hill FormulaC₁₆H₁₈ClN₃S * x H₂O
              Chemical formulaC₁₆H₁₈ClN₃S * x H₂O (x=2-3)
              Molar Mass319.86 g/mol (anhydrous)
              HS Code3204 13 00
              Structure formula Image
              Applications
              Biological Information
              Physicochemical Information
              Melting Point180 °C (decomposition)
              pH value3 (10 g/l, H₂O, 20 °C)
              Bulk density400 - 600 kg/m3
              Solubility50 g/l
              Dimensions
              Materials Information
              Toxicological Information
              LD 50 oralLD50 Rat 1180 mg/kg
              Safety Information according to GHS
              Hazard Pictogram(s)
              Hazard Statement(s)H302: Harmful if swallowed.
              Signal WordWarning
              Storage class10 - 13 Other liquids and solids
              WGKWGK 2 water endangering
              Disposal3
              Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
              Safety Information
              Hazard Symbols Harmful
              Categories of dangerharmful
              R PhraseR 22
              Harmful if swallowed.
              Product Usage Statements
              Storage and Shipping Information
              StorageStore at +5°C to +30°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Dye content (spectrophotometrically)≥ 82 %
              Identity
              - Chloride

              passes test
              - UV/VIS-Spectrumpasses test
              Absorption maximum λ max (ethanol 50 %)660 - 665 nm
              Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)2250 - 2750
              TLC-Testpasses test
              Loss on drying (110 °C)10 - 15 %
              Suitability for microscopypasses test

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              Product Number PackagingSpecificationLot Number
              1159430025 Glass bottlePDF Specification Document
              1159430100 Glass bottlePDF Specification Document

              Brochure

              Title
              Alles für die Histologie. Schnell und zuverlässig.
              Products for microscopy - All you need for successful working

              Reach Use

              Title
              Reach Use

              Instructions for Use

              Title
              115943 Azul de metileno (C.I. 52015) para microscopía Certistain®
              115943 Bleu de méthylène (C.I. 52015) pour la microscopie Certistain®
              115943 Blu di metilene (C.I. 52015) per microscopia Certistain®
              115943 Methylenblau (C.I. 52015) für die Mikroskopie Certistain®
              115943 Methylene blue (C.I. 52015) for microscopy Certistain®

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              Categories

              Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Redox Indicators
              IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes