Millipore Sigma Vibrant Logo

115935 Eosin Y (yellowish) (C.I. 45380)

View Products on Sigmaaldrich.com
115935
View Pricing & Availability

Overview

Replacement Information

Key Spec Table

CAS #EC NumberHill FormulaMolar Mass
17372-87-1241-409-6C₂₀H₆Br₄Na₂O₅691.88 g/mol
Description
Catalogue Number115935
DescriptionEosin Y (yellowish) (C.I. 45380)
OverviewEosin Y (C.I. 45380) is a CERTISTAIN® dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin Y staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange.
As the Eosin Y dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
CAS number17372-87-1
EC number241-409-6
Hill FormulaC₂₀H₆Br₄Na₂O₅
Molar Mass691.88 g/mol
HS Code3212 90 00
Structure formula ImageStructure formula Image
Quality LevelMQ400
Applications
Applicationfor microscopy Certistain®
Biological Information
Physicochemical Information
Density0.78 g/cm3 (978.5 hPa, 27.05 °C)
Flash point191.6 °C Not applicable
Melting Point295 - 296 °C
pH value6.55 (H₂O, 29 °C)
Bulk density710 kg/m3
Solubility300 g/l
Dimensions
Materials Information
Toxicological Information
LD 50 oralLD50 Rat > 2000 mg/kg
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H317: May cause an allergic skin reaction.
H319: Causes serious eye irritation.
Precautionary Statement(s)P261: Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P264: Wash skin thoroughly after handling.
P272: Contaminated work clothing should not be allowed out of the workplace.
P280: Wear protective gloves/ eye protection/ face protection.
P302 + P352: IF ON SKIN: Wash with plenty of water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Signal WordWarning
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of dangerirritant
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Dye content (gravimetrically)≥ 85 %
Identity (UV/VIS-Spectrum)passes test
Absorption maximum λ max (water)515 - 518 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water)1200 - 1400
TLC-Testpasses test
Loss on drying (110 °C)≤ 8 %
Suitability for microscopypasses test

Related Products & Applications

Alternative Products

Catalogue Number Description  
109249 Mayer's hemalum solution Show Pricing & Availability

Related Products

Catalogue Number Description  
102439 Eosin Y-solution 0.5% alcoholic Show Pricing & Availability
109249 Mayer's hemalum solution Show Pricing & Availability
109844 Eosin Y-solution 0.5% aqueous Show Pricing & Availability

Accessories (Secondary)

Catalogue Number Description  
115936 Erythrosine B (C.I. 45430) Show Pricing & Availability

Related Products By: Application Facete

Related Products By: Brand Facete

Categories

IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes
Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Fluorescence Indicators
Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Acid Alkali Indicators or pH Indicators