MOD50
Imprint® DNA Modification Kit
For bisulfite DNA conversion & purification
Synonym(s):
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
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About This Item
UNSPSC Code:
41116158
EC Number:
231-548-0
NACRES:
NA.54
Related Categories
General description
The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
Application
Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.
Features and Benefits
- Only 50 picograms of DNA or 20 cells are required
- Procedure takes less than 2 hours
- Greater than 99% conversion rate
- Extremely low degradation
- Option of convenient one-step protocol
- Consistent and reproducible Bisulfite Modification
- Can be used with genomic, endonuclease digested, and FFPE DNA
Preparation Note
All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Also Available Separately
Product No.
Description
SDS
- T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural
signalword
Danger
supp_hazards
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
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David E Condon et al.
BMC bioinformatics, 19(1), 31-31 (2018-02-07)
Identification of differentially methylated regions (DMRs) is the initial step towards the study of DNA methylation-mediated gene regulation. Previous approaches to call DMRs suffer from false prediction, use extreme resources, and/or require library installation and input conversion. We developed a
Hwan Young Lee et al.
International journal of legal medicine, 126(1), 55-62 (2011-06-01)
DNA analysis of various body fluid stains at crime scenes facilitates the identification of individuals but does not currently determine the type and origin of the biological material. Recent advances in whole genome epigenetic analysis indicate that chromosome pieces called
Elena Ivanova et al.
Clinical epigenetics, 12(1), 64-64 (2020-05-13)
Preimplantation embryos experience profound resetting of epigenetic information inherited from the gametes. Genome-wide analysis at single-base resolution has shown similarities but also species differences between human and mouse preimplantation embryos in DNA methylation patterns and reprogramming. Here, we have extended
Nelly Olova et al.
Genome biology, 19(1), 33-33 (2018-03-17)
Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some steps in the procedure, such
Masoumeh Afzali et al.
Iranian biomedical journal, 17(2), 77-83 (2013-04-10)
The protein of Niemann-pick type C1 (NPC1) gene promotes the egress of cholesterol from late endosomes and lysosomes to other cellular compartments and contributes to a process known as reverse cholesterol transport. This study aimed to examine whether promoter methylation
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