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Cell Isolation

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If you need high yields of pure, viable and functional cells, magnetic cell separation is the technology of choice. This method allows an easy isolation and characterization of many cell types such as: human, mouse or cells from other species.

Estapor® Paramagnetic Microspheres are reagents that allow for the extraction of specific cells or micro-organisms by means of a simple magnet. Linked to a molecule which is capable of recognizing a specific target, Estapor® Paramagnetic Microspheres are the best choice for cell separation. They allow a better yield, eliminate process steps, save time and promote cell viability.

Principle of this assay

  1. First, the mixture of cells to be separated is incubated with magnetic microspheres coated with antibodies against a particular surface antigen.

  2. Then, a coated magnetic microspheres-target cell complex appears.

  3. The cell solution is transferred to a column (or tube) then placed in the presence of a strong magnetic field. The complex stays in the column, while other cells flow through.
Cell Isolation

Estapor® has developed a specific expertise for attaching bio molecules to the surface of Paramagnetic Microspheres, and offers microspheres with the best size which is requiring in cell sorting.
In fact, using microspheres with a diameter > 1µm requires a step of detachment.
With our microspheres, this step is not required and allows a time-saving measure, a better cell viability.

In positive selection (Figure 2), cells of interest are selected directly by binding with coated Estapor® microspheres and selected by magnetization, while discarded supernatant containing other cells is eliminated.

Figure 2

In negative selection (Figure 3), the Estapor® microspheres are coated with antibodies against known antigens, not against cell of interest. After administration of the mixture and Estapor® coated microspheres, these antigens bind onto the beads. The supernatant is collected as it contains cells of interest with a high degree of purity and viability.

Figure 3