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Application Notes

Examples of How to Use SeQuant® Products to Solve Your Analysis Problems

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/LE-SeQuant ph-150x171-05082014.jpgThis collection of Application Notes has been produced by SeQuant® scientists or developed by customers to assist you in the analysis of your compound. Each application note focuses on separation of a specific compound or type of compound with recommendations for the best column and separation conditions to use. Examples of expected data are also included.

We take pride in helping to support your research success and encourage you to share your experience as we continuously strive to expand and share this resource collection.

Don’t see information about purification of your compound? Check our complete collection of technical support resources or try using our online HILIC Retention Prediction Tool. Just enter your molecular formula to determine the optimal conditions for purification on ZIC®-HILIC columns.

Visit our on-line tutorial to learn more about HILIC technology or request our comprehensive „A Practical Guide to HILIC“ booklet. Both provide detailed explanations of technology and techniques to ensure your success using Merck’s innovative SeQuant® ZIC®-HILIC products.

If you still can’t find the information you need, you can always contact the SeQuant® Support Team who are happy to assist you.

Application Compilations

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Brochures/Le-Small Molecule Bioanalysis-100x104-05082014.jpg
Small Molecule Bioanalysis by LC-MS and LC-MS/MS

Analysis of endogenous and exogenous compounds in biological samples

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Brochures/LE-Polar Hydrophilic Compounds-100x104-05082014.jpg
Polar Hydrophilic Compounds in Pharmaceutical Analysis – Part II

Monograph modernization using SeQuant® ZIC®

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Brochures/LE-Analysis of polar compounds cover-100x137-05082014.jpg
Food Safety and Food Quality Control

Analysis of Polar Compounds in Complex Samples
Applications for HPLC and LC-MS with SeQuant® ZIC®-HILIC columns

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Brochures/LE-Polar Hydrophilic Compounds-100x139-05082014.jpg
Polar Hydrophilic Compounds in Pharmaceutical Analysis

SeQuant® ZIC®-HILIC HPLC for control of active ingredients, impurities, counterions, and excipients.

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ZIC®-HILIC Application Notes
2700-06A ZIC®-HILIC Separation of Purines and Pyrimidines
2700-07A ZIC®-HILIC Separation of Methacrylamide, Acrylamide, Methacrylic Acid
2700-08A Volume Loadability of the ZIC®-HILIC Column 
2700-09A Scalability of Separations on the ZIC®-HILIC Column
2700-11A Retention Time Stability on the ZIC®-HILIC Column
2700-12A ZIC®-HILIC Separation of Dehydroascorbate and Ascorbic Acid
2700-13A ZIC®-HILIC Separation of Fumaric, Oxalic and Citric Acid
2700-14A ZIC®-HILIC Separation of Chlormequat and Mepiquat
2700-15A ZIC®-HILIC Separation of UMP, UDP and UTP
2700-17A ZIC®-HILIC Separation of N-Acetyl-L-Cysteine, Citrulline
2700-18A ZIC®-HILIC Separation of organophosphonate nerve agent metabolites
2700-19A ZIC®-HILIC Separation of gamma-Hydroxybutyric Acid
2700-20A ZIC®-HILIC Separation of 4-Amino-3-hydroxyphenylalanine and 3-Amino-4-hydroxyphenylalanine
2700-21A ZIC®-HILIC Separation of cAMP, AMP, ADT and ATP 
2700-22A ZIC®-HILIC Separation of 5-Fluorouracil and 5-Fluorouracil Dimer
2700-23A ZIC®-HILIC Separation of Caffeine and Theophylline 
2700-24A ZIC®-HILIC Separation of CMP CDP and CTP 
2700-25A ZIC®-HILIC Separation of GMP GDP and GTP 
2700-26A ZIC®-HILIC Separation of Pyridoxal 
2700-27A ZIC®-HILIC Separation of Morphine from its 3- and 6-Glucoronides
2700-28A Upscaling from a 4.6 mm to 20 mm ID ZIC®-HILIC Column 
2700-29A ZIC®-HILIC Separation of 1,5-Napthalenedisulphonic Acid 
2700-30A ZIC®-HILIC Separation of Betaine from Chinese Lycium 
2700-31A ZIC®-HILIC Separation of Moniliformin 
2700-32A ZIC®-HILIC Separation of Eflornithine 
2700-33A ZIC®-HILIC Separation of Urea 
2700-34A ZIC®-HILIC Separation of EDTA 
2700-35A ZIC®-HILIC Separation of Oseltamavir and Oseltamavir Carboxylate 
2700-36A ZIC®-HILIC Fast Separation of AMP and ATP 
2700-37A ZIC®-HILIC Batch-to-batch Variability of Semi Preparative ZIC®-HILIC Columns 
2700-38A ZIC®-HILIC Retention Factor vs Porosity 
2700-39A ZIC®-HILIC Selectivity vs Particle Type and Size 
2700-40A ZIC®-HILIC Separation of Choline and Inositol 
2700-41A ZIC®-HILIC Simultaneous Separation of Anions and Cations 
2700-42A ZIC®-HILIC Separation of Acetic Acid 
2700-43A ZIC®-HILIC Separation of Azacitidine 
2700-44A ZIC®-HILIC Separation of Risedronate 
2700-45A ZIC®-HILIC Separation of PTSA and Pyrimethamine 
2700-46A ZIC®-HILIC Separation of tryptic peptides from Ovalbumin 
2700-47A ZIC®-HILIC Separation of tryptic peptides from Bovine Serum Albumin (BSA cow) 
2700-48A ZIC®-HILIC Separation of tryptic peptides from Cytochrome C (horse) 
2700-49A ZIC®-HILIC Separation of Fructose and Sucrose 
2700-51A ZIC®-HILIC Separation of Sucrose with RI detection

Articles featuring separation using ZIC®-HILIC published in the journal LCGC

LCGC-EU April 2003 Separation of Morphine and Glucuronated Metabolites on a Bonded Zwitterionic Stationary Phase 
LCGC-EU March 2005 Mass Spectrometric Detection of Homocysteine, Methylmalonic Acid and Succinic Acid using HILIC on a Zwitterionic Stationary Phase 
LCGC-EU May 2004 Separation of Polar and Hydrophilic Compounds Using a Zwitterionic Stationary Phase in HILIC 
LCGC-NA Sept 2004 Separation of Polar and Hydrophilic Compounds Using a Zwitterionic Stationary Phase in HILIC 
LCGC-NA Aug 2006 Simultaneous Determination of Positive and Negative Counterions Using a Hydrophilic Interaction Chromatography Method

ZIC®-pHILIC Application Notes

2800-02B ZIC®-pHILIC Separation of Flavonoids
2800-03B ZIC®-pHILIC Separation of Adenosine Phosphates 
2800-04B ZIC®-pHILIC Separation of Aliphatic Amino Acids 
2800-05B ZIC®-pHILIC Separation of Fumaric Oxalic and Citric Acid 
2800-06B ZIC®-pHILIC Separation of Nucleotides using ZIC-pHILIC and Negative ESI-MS 
2800-07B ZIC®-pHILIC Separation of Purines and Pyrimidines 
2800-08B ZIC®-pHILIC Separation of Tartaric and Ascorbic Acid 
2800-09B ZIC®-pHILIC Separation of Angiotensin II Bradykinin and Gly-His-Lys 
2800-10B ZIC®-pHILIC Separation of Fructose Glucose Sucrose and Lactose 
2800-11B ZIC®-pHILIC Gradient Separation of Fructose Glucose Sucrose and Lactose 


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