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ABE2600-100UL Anti-DNA Helicase B (HELB)

Anti-DNA Helicase B (HELB) Antibody, Cat. No. ABE2600, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-DNA Helicase B (HELB): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Helb UniProt Number: Q6NVF4
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ABE2600-100UL
100 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MICC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2600-100UL
      DescriptionAnti-DNA Helicase B (HELB)
      Alternate Names
      • HELB
      Background InformationDNA helicase B (UniProt: Q6NVF4; also known as EC: 3.6.4.12, HELB) is encoded by the Helb gene (Gene ID: 117599) in murine species. HELB helicase B is is a predominantly nuclear helicase that can undergo phosphorylation at Serine 942 by CDK2 during the G1/S transition that results in its export into the cytoplasm as cells approach and progress through S phase. Its nuclear export signal lies within amino acids 1022-1046. It serves as a 5 -3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection. It is shown to bind to RPA1 and this interaction promotes HELB recruitment to chromatin following DNA damage. Following DNA damage it is recruited to sites of double-strand breaks by the Replication Protein A (RPA) complex. Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB. HELB is reported to act independently of TP53BP1. Loss of DNA helicase B results in PARP inhibitor resistance in BRCA1-deficient tumor cells.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH7.4 with 0.05% sodium azide.
      Applications
      ApplicationAnti-DNA Helicase B (HELB) Antibody, Cat. No. ABE2600, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected DNA Helicase B (HELB) in Immunocytochemistry applications (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).

      Western Blotting Analysis: A representative lot detected DNA Helicase B (HELB) in Western Blotting applications (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 227 amino acids from the C-terminal half of murine DNA helicase B.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects DNA helicase B in murine species. It targets an epitope within 227 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Helb
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight145 kDa observed; 121.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in wild-type mouse embryonic fibroblasts .

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DNA helicase B (HELB) in lysate from wild-type mouse embryonic fibroblasts (MEF) and MEF heterogygotes for HELB.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE2600-100UL 04054839468872