Millipore Sigma Vibrant Logo

ABN1446 Anti-GPRIN1

Anti-GPRIN1 Antibody, Cat. No. ABN1446, is a highly specific rabbit polyclonal antibody that targets GPRIN1 and has been tested in Immunohistochemistry and Western Blotting.

Research Category: Neuroscience Gene Symbol: GPRIN1, KIAA1893 UniProt Number: Q7Z2K8
View Products on Sigmaaldrich.com
ABN1446
50 µL  
Preis wird abgerufen...
Preis nicht abrufbar
Die Mindestmenge muss ein Vielfaches sein von
Maximum Quantity is
Bei Bestätigung Weitere Informationen
Sie haben () gespart
 
—
Bitte erfragen
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      Sonderaktionen

       

      Kontakt zum Kundenservice

      Übersicht

      Replacement Information

      Sonderaktionen

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1446
      DescriptionAnti-GPRIN1
      Alternate Names
      • G protein-regulated inducer of neurite outgrowth 1
      • GRIN1
      Background InformationG protein-regulated inducer of neurite outgrowth 1 (UniProt Q7Z2K8; also known as GRIN1) is encoded by the GPRIN1 (also known as KIAA1893) gene (Gene ID 114787) in human. Originally identified as a binding protein for guanosine 5'-3-O-(thio)triphosphate-bound G(z)alphaz, G protein-regulated inducer of neurite outgrowth 1 (GPRIN1) is a signaling molecule that functions as a downstream target of G(o)alpha. When co-expressed with constitutively active form of G(o)alpha, GPRIN1 is demonstrated to stimulate neurite extensions in Neuro2a cells. Gprin1 expression in mice is limited to the nervous system at all developmental stages, occurring within the entire embryonic mantle zones and becoming restricted to sets of nuclei at postnatal to adult stages. Gprin1 immunoreactivity is found colocalized with G(o)alpha at neuronal dendrites and axons, while being undetected in glial cells. Studies conducted in rat reveals that 7 nicotinic receptor ( 7) significantly reduces axon growth in hippocampal neurons by coupling to Gprin1, which targets it to the growth cone. RNAi-mediated Gprin1 knockdown abolishes the localization and calcium signaling of 7 at the growth cone. The 7/Gprin1 interaction appears intimately linked to a G(o)alpha, growth-associated protein 43, and CDC42 cytoskeletal regulatory pathway within the developing axon.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GPRIN1 Antibody, Cat. No. ABN1446, is a highly specific rabbit polyclonal antibody that targets GPRIN1 and has been tested in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GPRIN1 in human prostate cancer, cerebral cortex and pancreas tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence of human GPRIN1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody targets an internal sequence present in both spliced isoforms of human GPRIN1 protein reported by UniProt (Q7Z2K8).
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GPRIN1
      • KIAA1893
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~105 kDa observed. 102.4 kDa (isoform 1) and 80.95 kDa (isoform 2) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HT-29 cell lysate.

      Western Blotting Analysis: A 1:20,000 dilution of this antibody detected GPRIN1 in 10 µg of HT-29 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABN1446 04054839056291

      Documentation