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MABC1556 Anti-NRF2 Antibody, clone 6H11.1

Detect NRF2 using this mouse monoclonal Anti-NRF2 Antibody, clone 6H11.1, Cat. No. MABC1556. It has been used in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-NRF2 Antibody, clone 6H11.1: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Apoptosis & Cancer Gene Symbol: NFE2L2, NRF2 UniProt Number: Q16236
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MABC1556
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1556
      DescriptionAnti-NRF2 Antibody, clone 6H11.1
      Alternate Names
      • NFE2L2
      • Nuclear factor erythroid 2-related factor 2
      • NF-E2-related factor 2
      • NFE2-related factor 2
      • HEBP1
      Background InformationNuclear factor erythroid 2-related factor 2 (UniProt: Q16236; also known as NF-E2-related factor 2, NFE2-related factor 2, HEBP1) is encoded by the NFE2L2 (also known as NRF2) gene in human. It is widely expressed with high expression in adult muscle, kidney, lung, liver and in fetal muscle. NFE2L2 is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes and is essential for the coordinated up-regulation of genes in response to oxidative stress. It contains an acidic activation domain in the N-terminus and DNA binding domain in the C-terminus. NFE2L2 is usually localized in the cytoplasm under unstressed conditions, but translocates to the nucleus when subjected to induction by electrophilic agents. Phosphorylation of Ser40 by protein kinase C in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, thereby promoting its translocation into the nucleus. Acetylation at Lys596 and Lys599 increases its nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence. NFE2L2 is shown to be down-regulated by ENC1 via a proteasomal ubiquitin-independent protein catabolic process.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect NRF2 using this mouse monoclonal Anti-NRF2 Antibody, clone 6H11.1, Cat. No. MABC1556. It has been used in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NRF2 in human liver, human small intestine, human tonsil, and human pancreas.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Nuclear factor erythroid 2-related factor 2 (NRF2).
      Clone6H11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6H11.1 detects NRF2 in HepG2 cells. It targets an epitope within 15 amino acids in the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NFE2L2
      • NRF2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~64 kDa observed, 67.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected NRF2 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABC1556 04054839178962