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MABE878 Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3

This Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 is validated for use in western blotting & ICC for the detection of RNA Helicase A/Dhx9.

Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Dhx9, Ddx9 UniProt Number: O70133
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MABE878
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE878
      DescriptionAnti-RNA Helicase A/Dhx9 Antibody, clone 8E3
      Alternate Names
      • ATP-dependent RNA helicase A
      • RHA
      • DEAH box protein 9
      • mHEL-5
      • Nuclear DNA helicase II
      • NDH II
      Background InformationRNA Helicase A, also known as DEAH box protein 9 (mHEL-5), DHX9, or Nuclear DNA helicase II (NDH II), and encoded by the mouse gene Dhx9, is a component of the CRD-mediated mRNA stability complex. The CRD-mediated complex is a protein complex that binds to, and promotes stabilization of, mRNA molecules containing the coding region instability determinant (CRD). In humans, it consists of IGF2BP1 and at least four additional proteins: HNRNPU, SYNCRIP, YBX1, and RNA Helicase A. RNA Helicase A operates by unwinding dsDNA and RNA in a 3' to 5' direction. Additionally, RNA Helicase A acts as a transcriptional activator and interacts with LARP6 which is a protein that has been associated with fasting glucose traits, type 2 diabetes and obesity as well as the stabilization of collagen mRNAs for CO1A1 and CO1A2. RNA Helicase A is localized to the nucleolus within the nucleus but can also be found in the cytoplasm localized in cytoplasmic mRNP granules containing untranslated mRNAs and thus the protein shuttles between the nucleus and cytoplasm depending upon the microenvironment.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 is validated for use in western blotting & ICC for the detection of RNA Helicase A/Dhx9.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cell lysate (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).
      Immunocytochemistry Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cells (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of mouse RNA Helicase A/Dhx9.
      EpitopeN-terminus
      Clone8E3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Dhx9
      • Ddx9
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~140 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected RNA Helicase A/Dhx9 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE878 04053252946028

      Documentation

      Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 Analysenzertifikate

      TitelChargennummer
      Anti-RNA Helicase A/Dhx9, clone 8E3 - Q2367874 Q2367874
      Anti-RNA Helicase A/Dhx9, clone 8E3 - 3258758 3258758
      Anti-RNA Helicase A/Dhx9, clone 8E3 - 3693962 3693962
      Anti-RNA Helicase A/Dhx9, clone 8E3 -2790047 2790047
      Anti-RNA Helicase A/Dhx9, clone 8E3 -2791165 2791165
      Anti-RNA Helicase A/Dhx9, clone 8E3 -2821204 2821204
      Anti-RNA Helicase A/Dhx9, clone 8E3 -2846345 2846345
      Anti-RNA Helicase A/Dhx9, clone 8E3 -2888511 2888511
      Anti-RNA Helicase A/Dhx9, clone 8E3 -2892484 2892484
      Anti-RNA Helicase A/Dhx9, clone 8E3 Monoclonal Antibody 2930958

      Literatur

      ÜbersichtPub Med ID
      Monoclonal antibody specific for Dhx9/NDHII/RHA.
      Kotani, Manato, et al.
      Hybridoma (Larchmt), 29: 259-61 (2010)  2009

      Abstract anzeigen       		20569003 20569003