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ABT1760 Anti-phospho-VE-Cadherin (Tyr685)

This rabbit polyclonal Anti-phospho-VE-Cadherin (Tyr685), Cat. No. ABT1760 is validated for use in Western Blotting with immunogen peptide blocking for the detection of VE-cadherin tyrosine 685 phosphorylation.

Anti-phospho-VE-Cadherin (Tyr685): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Cell Structure Gene Symbol: CDH5 UniProt Number: P33151
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ABT1760
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT1760
      DescriptionAnti-phospho-VE-Cadherin (Tyr685)
      Alternate Names
      • Cadherin-5, Tyr685-phosphorylated
      • 7B4 antigen, Tyr685-phosphorylated
      • CD144, Tyr685-phosphorylated
      • Vascular endothelial cadherin, Tyr685-phosphorylated
      • VE-Cad, Tyr685-phosphorylated
      • VE-cadherin, Tyr685-phosphorylated
      Background InformationCadherin-5 (UniProt P33151; also known as 7B4 antigen, CD144, Vascular endothelial cadherin, VE-Cad, VE-cadherin) is encoded by the CDH5 gene (Gene ID 1003) in human. Cadherins (calcium-dependent adhesion) are type-1 transmembrane proteins that form adherens junctions in tissues and play important roles in mediating cell adhesion. Cadherins are designated with a prefix that specifies their tissue association. Vascular endothelial-cadherin (VE-cadherin) is the transmembrane component of the endothelial adherens junction between vascular endothelial cells (ECs) and plays a pivotal role in endothelium integrity and in the control of vascular permeability. One characteristic of VEGF-induced vascular permeability is the phosphorylation of VE-cadherin by Src family kinases (SFK), which leads to VE-cadherin internalization and the destabilization of adherens junctions. Likewise, VE-cadherin overexpression is shown to decrease the permeability of endothelial monolayers in vitro. SFK activation by dominant negative Csk overexpression is reported to result in VE-cadherin phosphorylation at tyrosines 658, 685, and 731 in human dermal microvascular endothelial cells (HDMECs) without affecting HDMEC monolayer permeability. On the other hand, expression of constitutively active Src promoted VE-cadherin phosphorylation on tyrosines 658 and 731 and decreased barrier function, suggesting that concurrent signaling events in addition to VE-cadherin tyrosine phosphorylation are needed to promote barrier permeability in response to inflammatory mediators or growth factors. VE-cadherin is initially produced with a signal peptide (a.a. 1-24) and a propeptide (a.a. 25-45) sequence, the removal of which yields tthe mature protein containing a large extracellular region (a.a. 46-599) with five cadherine repeats (a.a. 46-149, 150-256, 257-371, 372-476, 477-593), followed by a transmembrane segment (a.a. 600-620) and a cytoplasmic domain (a.a. 621-784).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-phospho-VE-Cadherin (Tyr685), Cat. No. ABT1760 is validated for use in Western Blotting with immunogen peptide blocking for the detection of VE-cadherin tyrosine 685 phosphorylation.
      Key Applications
      • Western Blotting
      Application NotesPeptide Inhibition Analysis: Target specificity of a representative lot was verified by immunogen peptide blocking in Western blotting application. Preblocking of the antibody with the immunogen peptide, but not the corresponding non-phosphorylated peptide, prevented target band detection.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr685 of human VE-cadherin isoform 1 precursor sequence.
      Epitopecytoplasmic domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityTarget specificity of this rabbit polyclonal antibody has been verified by immunogen peptide blocking in Western blotting application. Numbering of the target VE-cadherin phosphotyrosine residue varies depending on whether the N-terminal signal peptide sequence and propeptide sequence are included, as well as the species and spliced isoform a particular numbering is based on. Target pTyr this rabbit polyclonal detects corresponds to pTyr685/pTyr638 of human spliced isoform 1 precursor/mature sequence (UniProt P33151-1), pTyr570/pTyr523 of human spliced isoform 2 precursor/mature sequence (UniProt P33151-2), pTyr685/pTyr640 of bovine precursor/mature sequence (UniProt Q6URK6), and pTyr684/pTyr40 of porcine precursor/mature sequence (UniProt O02840). Equivalent target site sequence is not present in mouse, rat, or chicken VE-cadherin sequence.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine and Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDH5
      Modifications
      • Phosphorylation
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~130 kDa observed. Target band size appears larger than the calculated molecular weights of 82,58/69.55 kDa (human mature isoform 1/2), 87.53/74.50 kDa (human isoform 1/2 precursor), 82.80/87.47 kDa (bovine mature/precursor), and 82.45/87.55 kDa (porcine mature/precursor) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HUVEC lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected VE-cadherin pTyr685 phosphorylation in 10 µg of HUVEC lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABT1760 04054839057946