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345811 Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli, hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures.

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      Übersicht

      Replacement Information
      Description
      OverviewRecombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
      Catalogue Number345811
      Brand Family Calbiochem®
      References
      ReferencesMurata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
      Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
      Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
      Scudder, P., et al. 1983. Biochem. J. 213, 485.
      Product Information
      Activity≥14 U/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
      EC number3.2.1.103
      FormLiquid
      FormulationSterile-filtered in 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥140 U/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsThere is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      345811 0

      Documentation

      Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli Analysenzertifikate

      TitelChargennummer
      345811

      Literatur

      Übersicht
      Murata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
      Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
      Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
      Scudder, P., et al. 1983. Biochem. J. 213, 485.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-June-2007 JSW
      DescriptionRecombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
      FormLiquid
      FormulationSterile-filtered in 20 mM Tris-HCl, pH 7.5.
      Recommended reaction conditions
      Activity Assay
      Required Reagents • 5X Reaction Buffer: 250 mM sodium phosphate, pH 5.8 Assay For Glycoproteins 1. Add up to 100 µg glycoprotein to a clean tube. 2. Add 4 µl 5X Reaction Buffer. 3. Add H2O to a total volume of 19 µl. 4. Add 1 µl endo-β-galactosidase. 5. Incubate at 37°C for 2 h. For Oligosaccharides Follow the assay as outlined above in steps 1-4. Incubate at 37°C for several h to several days, depending on the substrate.
      EC number3.2.1.103
      Specific activity≥140 U/mg protein
      Activity≥14 U/ml
      Unit definitionOne unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsThere is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
      Toxicity Standard Handling
      ReferencesMurata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
      Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
      Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
      Scudder, P., et al. 1983. Biochem. J. 213, 485.