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IPFL00010 Immobilon-FL PVDF, 0,45 µm, 26,5 cm x 3,75 m-Rolle

Western Blotting, Dot/Slot Blotting

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Hauptanwendungen: Western Blotting, Dot Blot
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IPFL00010
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      Übersicht

      Replacement Information

      Key Spec Table

      Hauptanwendungen
      WB, DB
      Beschreibung
      BestellnummerIPFL00010
      Markenname
      • Immobilon
      BeschreibungImmobilon-FL PVDF, 0,45 µm, 26,5 cm x 3,75 m-Rolle
      HintergrundinformationenOverview
      - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
      Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

      Immobilon-P Transfer Membrane:
      - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
      - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

      Blotting Sandwiches:
      - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

      Blotting Filter Paper:
      - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

      Immobilon-PSQ Membrane:
      - 0.2 µm pore size and a large internal structure
      - Higher protein adsorption and sequencing yields than other membranes
      - Recommended for blotting proteins less than 20 kDa
      - Prevents blow-through of low molecular weight proteins

      Immobilon-FL Membrane:
      - The first transfer membrane optimized for fluorescence applications
      - Extremely low background improves sensitivity of all fluorescence detection protocols
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      - Ideal for multiplexing and chemifluorescence applications

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

      Features & Benefits:
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be reprobed multiple times

      Applications:
      - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
      - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      Literatur
      Produktinformationen
      NachweismethodeFluoreszent Chemifluoreszent Chromogen Chemiluminesenz 125I Fluoreszent
      Kompatible Färbemittellösungen
      • Alle Fluoreszenz-Farbstoffe
      • Coomassie-Brilliant-Blue
      • Amidoschwarz
      • Ponceau-S-Rot
      • CPTS
      • Sypro®-Ruby-Farbstoff
      FiltercodeIPFL
      FilterfarbeWeiß
      Quality LevelMQ400
      Anwendungen
      AnwendungWestern Blotting, Dot/Slot Blotting
      Hauptanwendungen
      • Western Blotting
      • Dot Blot
      Biologische Informationen
      Rinderserumalbumin-Adsorption205 µg/cm²
      Ziegen-IgG-Adsorption300 µg/cm²
      Insulin-Adsorption155 µg/cm²
      BenetzbarkeitHydrophob
      Physiochemische Informationen
      Porengröße0.45 µm
      Abmessungen
      FilteroberflächeGlatt
      Filterabmessungen27 cm x 3.75 m
      Materialinformationen
      Chemie
      • Hydrophobes PVDF
      Angaben zur Toxikologie
      Sicherheitsinformationen gemäß GHS
      Sicherheitshinweise
      Hinweise zum Produktgebrauch
      Lagerungs- und Versandinformationen
      Verpackungsinformationen
      Menge1
      Transportinformationen
      Ergänzende Informationen
      Spezifikationen
      Global Trade Item Number
      Bestellnummer GTIN
      IPFL00010 04053252669439

      Documentation

      Immobilon-FL PVDF, 0,45 µm, 26,5 cm x 3,75 m-Rolle SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Broschüre

      Titel
      SNAP i.d. 2.0 System Brochure
      Western Blotting Tools

      Technische Informationen

      Titel
      Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system
      Protein Blotting Handbook: 6th Edition

      Posters

      Titel
      Low Background Membrane for Fluorescent Protein Detection in Western Blotting

      Gebrauchsanleitungen

      Titel
      Immobilon® Block Noise Cancelling Reagents
      Immobilon®-FL Transfer Membrane User Guide
      User Guide - Immobilon® FL Transfer Membrane