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MABN2439 Anti-ABCA4 Antibody, clone 3F4

Anti-ABCA4, clone 3F4, Cat. No. MABN2439, is a highly specific mouse monoclonal antibody that targets ABCA4 (RIM) and has been tested in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, and Western Blotting.

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MABN2439
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2439
      DescriptionAnti-ABCA4 Antibody, clone 3F4
      Alternate Names
      • ABC transporter A4
      • RIM ABC Transporter
      • RIM protein
      Background InformationRetinal-specific ATP-binding cassette transporter (UniProt F1MWM0/P78363; also known as ATP-binding cassette sub-family A member 4, ABC transporter A4, RIM ABC transporter, RIM Protein) is encoded by the ABCA4 gene in bovine species. ABC transporter A4, a ~250 kDa single-chain, is a multi-pass membrane protein of the superfamily of ATP-binding cassette (ABC) transporters that is localized to the disk margins of vertebrate photoreceptor outer segments. It is implicated in the clearance of all-trans retinal derivatives from rod and cone photoreceptor cells following photoexcitation as part of the visual cycle. ABC transporter A4 is composed of two symmetrically organized halves, each comprising six membrane-spanning helices, a large glycosylated exocytoplasmic domain located inside the disk, and a cytoplasmic domain with an ATP-binding cassette. Mutations in ABCA4 are known to cause Stargardt macular degeneration, which is an autosomal recessive form of juvenile macular degeneration associated with a severe loss in vision. (Ref.: Tsybovsky, Y et al. (2010). Adv. Exp. Med. Biol. 703, 105-125).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ABCA4, clone 3F4, Cat. No. MABN2439, is a highly specific mouse monoclonal antibody that targets ABCA4 (RIM) and has been tested in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A 1:50 dilution from a representative lot detected ABCA4 in mouse retina cells.

      Immunofluorescence Analysis: A 1:250 dilution from a representative lot detected ABCA4 in mouse retina cells (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

      Immunoprecipitation Analysis: A representative lot detected ABCA4 in Immunoprecipitation applications (Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

      Western Blotting Analysis: A representative lot detected ABCA4 in Western Blotting applications (Molday, L.L., et. al. (2000). Nat Genet. 25(3):257-8; Illing, M., et. al. (1997). J Biol Chem. 272(15):10303-10; Beharry, S., et. al. (2004). J Biol Chem. 279(52):53972-9).

      Immunohistochemistry Analysis: A representative lot detected ABCA4 in Immunohistochemistry applications (Molday, L.L., et. al. (2000). Nat Genet. 25(3):257-8).
      Biological Information
      ImmunogenWhole purified bovine disk rim protein.
      Epitopedomain of:
      Clone3F4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3F4 detects ABC transporter (ABCA4) in Bovine and Human cells. It targets an epitope within 9 amino acids from the C-terminal end.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Bovine
      • Mouse
      Species Reactivity NoteHuman, Bovine, Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ABCA4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~220 kDa observed; 257.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human retina tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected ABCA4 in 10 µg of human retinal tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2439 04054839337192

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