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MABF2007-25UG Anti-CHIKV E2 Antibody, clone 1.3A2

Anti-CHIKV E2, clone 1.3A2, Cat. No. MABF2007, is a mouse monoclonal antibody that detects E2 protein of Chikungunya virus and has been tested for use in ELISA, Neutralization, and Western Blotting.

MABF2007-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2007-25UG
      DescriptionAnti-CHIKV E2 Antibody, clone 1.3A2
      Alternate Names
      • E2 Protein CHIKV
      • Chikungunya virus E2 protein
      Background InformationChikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes. Immune compromised individuals may face serious complications, including encephalitis and mortality. CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K. The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion. The ectodomain E1 protein consists of three domains known as D1, DII, and DIII. The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker. Both E1 and E2 proteins are responsible for virus entry into host cells. The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions. Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating. This monoclonal antibody (clone 1.3A2) can recognize E2 protein in unreduced samples, but not in DTT-reduced/carboxymethylated samples. It can also effectively neutralize different CHIKV isolates (CHIKV- MAU and CHIKV-ASIAN). (Ref.: Goh, LYH et al., (2013). Clin. Immunol. 149(3); 487-497).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Applications
      ApplicationAnti-CHIKV E2, clone 1.3A2, Cat. No. MABF2007, is a mouse monoclonal antibody that detects E2 protein of Chikungunya virus and has been tested for use in ELISA, Neutralization, and Western Blotting.
      Key Applications
      • ELISA
      • Neutralization Assay
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected E2 of Chikungunya virus in C6/36 mosquito cell lysates either mock-infected (negative) or CHIKV-infected (positive). (Courtesy of Jody Peters, Ph.D., University of Queensland, Australia).

      Neutralizing Analysis: A representative lot efficiently neutralized different CHIKV isolates in Neutralizing applications (Goh, L.Y., et. al. (2013). Clin Immunol. 149(3):487-97).

      Western Blotting Analysis: A representative lot detected CHIKV E2 in Western Blotting applications (Goh, L.Y., et. al. (2013). Clin Immunol. 149(3):487-97).

      ELISA Analysis: A representative lot detected CHIKV E2 in ELISA applications (Goh, L.Y., et. al. (2013). Clin Immunol. 149(3):487-97).
      Biological Information
      ImmunogenSucrose gradient-purified, gamma-irradiated CHIKVMAU E2 protein (Mauritius strain 06113879) with Quil-A saponin as adjuvant followed by a second application and later a boost with binary-ethyleneimine inactivated CHIKVASIAN.
      Clone1.3A2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1.3A2 specifically detects E2 glycoprotein in Chikungunya virus. May recognize multiple epitopes on E2 protein.
      IsotypeIgG2aκ
      Species Reactivity
      • Virus
      Species Reactivity NoteChikungunya virus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed to be mouse IgG2a by isotype testing.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2007-25UG 04054839646706