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MABF1988-100UG Anti-CMTM6 Antibody, clone RCT6

Anti-CMTM6, clone RCT6, Cat. No. MABF1988, is a mouse monoclonal antibody that detects CMTM6 and is tested for use in Immunohistochemistry and Western Blotting.

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MABF1988-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF1988-100UG
      DescriptionAnti-CMTM6 Antibody, clone RCT6
      Alternate Names
      • CKLF-like MARVEL transmembrane domain-containing protein 6
      • Chemokine-like factor superfamily member 6
      Background InformationCKLF-like MARVEL transmembrane domain-containing protein 6 (UniProt: Q9NX76; also known as Chemokine-like factor superfamily member 6, CMTM6) is encoded by the CMTM6 gene (Gene ID: 54918) in human. CMTM6 is a ubiquitously expressed, multi-pass membrane protein of the family of MARVEL domain-containing proteins with 3 cytoplasmic domains, 4 transmembrane domains, and 2 extracellular domains. Its MARVEL domain is localized in amino acids 33-160. It serves as a master regulator of recycling and plasma membrane expression of PD-L1. It binds to PD-L1 and maintains its cell surface expression. Although it is not required for PD-L1 maturation, but it co-localizes with PD-L1 at the plasma membrane and in recycling endosomes, where it prevents PD-L1 from being targeted for lysosome-mediated degradation by preventing its STUB-1 mediated ubiquitination. It also stabilizes PD-L1 expression on antigen presenting cells and potentiates inhibitory signaling by PD-1 (CD279), ultimately triggering T-cell anergy. It enhances the ability of PD-L1-expressing tumor cells to inhibit T cell activity. (Ref.: Burr, ML., et al. (2017). Nature. 549(7670); 101-105; Mezzadra, R., et al. (2017). Nature. 549(7670); 106-110).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CMTM6, clone RCT6, Cat. No. MABF1988, is a mouse monoclonal antibody that detects CMTM6 and is tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected CMTM6 in Immunohistochemistry applications (Mezzadra, R., et al. (2017). Nature. 549(7670):106-110).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA linear peptide corresponding to a sequence from the C-terminal region of human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6).
      EpitopeC-terminal
      CloneRCT6
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone RCT6 is a mouse monoclonal antibody that detects human CMTM6. It targets an epitope within the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CMTM6
      Purification MethodPurified
      UniProt Number
      Molecular Weight~20 kDa observed; 20.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western blotting in lysates from 8505c cells overexpressing CMTM6.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CMTM6 in lysates from 8505C cells overexpressing CMTM6, but not in knockout or untransfected cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF1988-100UG 04065267061696