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MABS2153-100UG Anti-Cep55 Antibody, clone 5A3

Anti-Cep55, clone 5A3, Cat. No. MABS2153, is a mouse monoclonal antibody that detects Centrosomal protein of 55 kDa (Cep55) and has been tested for use in Western Blotting.

MABS2153-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2153-100UG
      DescriptionAnti-Cep55 Antibody, clone 5A3
      Alternate Names
      • Centrosomal protein of 55 kDa
      • Up-regulated in colon cancer 6
      • FLJ10540
      Background InformationCentrosomal protein of 55 kDa (UniProt: Q53EZ4; also known as Cep55, Upregulated in colon cancer 6) is encoded by the CEP55 (also known as C10orf3, URCC6) gene (Gene ID: 55165) in human. Cep55 is a homodimeric protein that plays a role in mitotic exit and successful completion of cytokinesis. It is located in the centrosome in interphase cells and recruited to the midbody during cytokinesis. Cep55 is highly expressed in testis and its expression is also reported in fetal brain ganglionic eminence, kidney tubules, and multinucleate neurons in the temporal cortex. Its expression has also been reported in several cancer cell lines. Cep55 is present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas most of it localizes to the pericentriolar material. During mitosis, it loses its affinity for the centrosome at the onset of prophase and diffuses throughout the cell. This dissociation from the centrosome is phosphorylation dependent. In Cep55, serine 425 and 428 are shown to be phosphorylated at the onset of mitosis and prior to serine 436 phosphorylation. These phosphorylations are essential for dissociation from the centrosome at the G2/M boundary. In Western blot analysis of synchronized cells, Cep55 may appear as a doublet that is indicative of phosphorylation during mitosis, and a single protein in interphase cells. In cancer cells, Cep55 expression is correlated with cell growth rate and p53 is shown to negatively regulate its levels and promoter activity. Down-regulation of Cep55 is accompanied by repression of polo-like kinase 1 (PLK1). Over-expression of PLK1 and knockdown of p53 are reported to enhance the post-translational stability of Cep55. (Ref.: Chang, YC., et al. (2010). J. Cell. Biochem. 109(6); 1274 1285; Chang, YC., et al. (2012). J. Biol. Chem. 287(6); 4376-4385).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Cep55, clone 5A3, Cat. No. MABS2153, is a mouse monoclonal antibody that detects Centrosomal protein of 55 kDa (Cep55) and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected Cep55 in HeLa cell lysate.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant human Centrosomal protein of 55 kDa (Cep55).
      EpitopeUnknown
      Clone5A3
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 5A3 is a mouse monoclonal antibody that detects Centrosomal protein of 55 kDa (Cep55).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CEP55
      • C10orf3
      • URCC6
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~55 kDa observed; 54.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 1:1,000 dilution of this antibody detected Cep55 in Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2153-100UG 04061841879671