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MABT528-AF488 Anti-Connexin 43 Antibody, CT Antibody, clone 1C5.1 Antibody, Alexa Fluor® 488 Conjugate

This mouse monoclonal Anti-Connexin 43 Antibody, CT, clone 1C5.1, Alexa Fluor® 488 Conjugate, Cat. No. MABT528-AF488 is used for the detection of Connexin 43 by Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: GJA1, GJAL UniProt Number: P17302
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MABT528-AF488
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMABT528-AF488
      DescriptionAnti-Connexin 43 Antibody, CT Antibody, clone 1C5.1 Antibody, Alexa Fluor® 488 Conjugate
      Alternate Names
      • Gap junction alpha-1 protein
      • Cx43
      • Gap junction 43 kDa heart protein
      Background InformationGap junction alpha-1 protein (UniProt: P17302; also known as Connexin-43, Cx43, Gap junction 43 kDa heart protein) is encoded by the GJA1 (also known as GJAL) gene (gene ID: 2697) in human. Cx43 is a member of the connexin family of proteins and a component of gap junctions. Connexins belong to a family of integral membrane proteins that assemble as hexamers (connexons) to form gap junctions, which are specialized sites of cell-cell contact. Gap junctions allow the passage of ions, metabolites and low molecular weight messenger molecules to pass from one cell to the other. Cx43 undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Cx43 may play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. It also acts as a negative regulator of bladder functional capacity by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic stimuli and causing them to contract. Cx43 is reported to be essential for normal cardiac development. Mice lacking Cx43 die soon after birth due to cardiac malformations and obstruction of the pulmonary artery. Cx43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea, and testis. Cx43 is phosphorylated at Ser368 by PKC gamma; which leads to disassembly of gap junction plaques and inhibition of gap junction activity. Mutations in GJA1 gene are reported to cause Oculodentodigital dysplasia and Hallermann-Streiff syndrome.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse monoclonal antibody in PBS with 1.5% BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-Connexin 43 Antibody, CT, clone 1C5.1, Alexa Fluor® 488 Conjugate, Cat. No. MABT528-AF488 is used for the detection of Connexin 43 by Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. MABT528) is shown to be suitable also for Flow Cytometry, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation applications.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 68 amino acids from the C-terminal half of human Connexin 43.
      Clone1C5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityAnti-Connexin 43, CT antibody, clone 1C5.1, Alexa Fluor® 488 Conjugate specificaly detects Connexin 43 in human and rat. This antibody has been shown to bind to an epitope between amino acids 315-382.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteRat, Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GJA1
      • GJAL
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight43.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Connexin 43 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT528-AF488 04054839167539