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MABC1112 Anti-Diasialoganglioside GD3 Antibody, clone R24

Detect ganglioside GD3 using this mouse monoclonal Anti-Diasialoganglioside GD3, clone R24 Antibody, Cat. No. MABC1112, validated for use in Flow Cytometry and Immunocytochemistry, Western Blotting, Affects Function, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry, Affinit

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Research Category: Apoptosis & Cancer
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MABC1112
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MFUNC, FC, IHC, IH(P), IP, WB, ABAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1112
      DescriptionAnti-Diasialoganglioside GD3 Antibody, clone R24
      Alternate Names
      • GD3
      • Ganglioside GD3
      • Disialoganglioside GD3
      Background InformationGangliosides are sialic acid-containing oligoglycosylceramides. They are classified based on the number of sialic acid residues per molecule. GM gangliosides contain one sialic acid residue, GD gangliosides contain two, GT contain three, and GQ contain four sialic acid residues. Sialyltransferase-catalyzed 2-3 sialylation of the galactose of the trisaccharide Gal 1-4Glc 1-1 Cer produces GM3, which can be further sialylated to produce GD3 with two sialic acid on the galactose. GD3 is found in melanocytes and other cells that originate in the neuroectoderm. It is expressed almost exclusively among gangliosides in the early stage of the brain development of mice and rats. It is also detected in the T cell malignant leukocytes, such as acute lymphoblastic leukemia cells, adult T cell leukemia cells, and in activated normal human T lymphocytes. GD3 is found in almost all melanomas and is considered as an important biomarker for malignant melanoma. GD3 positive cells show markedly increased cell growth and invasive characteristics. Suppression of GD3 expression with antisense GD3 synthase cDNA is shown to reduce tumor cell phenotypes such as growth, migration, metastasis, and angiogenesis in F11 cells. Intra-cytoplasmic domain of neogenin is reported to act as a driver to promote various cancer phenotypes. Ref.: Hamamura, K et al. (2005). Proc. Natl. Acad. Sci. USA. 102 (31), 11041 11046; Dippold, WG et al. (1985). Cancer Res. 45(8): 3699-3705; Kaneko, K et al (2016). J. Biol. Chem. (In press).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG3 in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect ganglioside GD3 using this mouse monoclonal Anti-Diasialoganglioside GD3, clone R24 Antibody, Cat. No. MABC1112, validated for use in Flow Cytometry and Immunocytochemistry, Western Blotting, Affects Function, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry, Affinit
      Key Applications
      • Affects Function
      • Flow Cytometry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      • Affinity Binding Assay
      Application NotesAffects Function: A representative lot induced cellular Lyn kinase activity and tyrosine phosphorylation of cellular proteins of primary rat cerebellar cultures in a time-dependent manner (Kasahara, K., et al. (1997). J. Biol. Chem. 272(47):29947-29953).

      Affinity Binding Assay: A representative lot reacted with heat stable glycolipid on the surface of all SK-MEL melanoma and two of the five astrocytoma cells tested, whereas epithelial cell types, fibroblasts, and cells of hematopoietic origin were found to lack the surface antigen recognized by clone R24 (Dippold, W.G., et al. (1980). Proc. Natl. Acad. Sci. U.S.A. 77(10):6114-6118).

      Flow Cytometry Analysis: A representative lot immunostained the surface of cultured primary rat cerebellar cells (Kasahara, K., et al. (1997). J. Biol. Chem. 272(47):29947-29953).

      Immunocytochemistry Analysis: A representative lot immunostained NGF-induced nurite outgrowths of cultured DRG neurons from wild-type, but not GD3 synthase-knockout, mice (Ribeiro-Resende, V.T., et al. (2014). PLoS One. 9(10):e108919).

      Immunohistochemistry Analysis: A representative lot detected GD3 immunoreactivity in unfixed frozen tissue samples of primary melanoma and metastatic malignant melanoma (Dippold, W.G., et al. (1985). Cancer Res. 45(8):3699-3705).

      Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Lyn from rat brain membrane extract. Clone R24 co-immunoprecipitated caveolin and the exogenously expressed Lyn, but not Src, from CHO cells expressing exogenous GD3 synthase (Kasahara, K., et al. (1997). J. Biol. Chem. 272(47):29947-29953).
      Biological Information
      ImmunogenSK-MEL-28 human melanoma cells (Dippold, W.G., et al. (1980). Proc. Natl. Acad. Sci. U.S.A. 77(10):6114-6118).
      CloneR24
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone R24 (a.k.a. R-24) immunostained neurites of cultured DRG neurons from wild-type, but not GD3 synthase-knockout, mice (Ribeiro-Resende, V.T., et al. (2014). PLoS One. 9(10):e108919).
      IsotypeIgG3κ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteTarget glycolipid is not species-specific.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry Analysis in SK-MEL-28 human skin melanoma cells.

      Flow Cytometry Analysis: 0.2 µL of this antibody detected GD3 immunoreactivity on the surface of one million SK-MEL-28 human skin melanoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1112 04054839139178

      Documentation

      Anti-Diasialoganglioside GD3 Antibody, clone R24 Certificates of Analysis

      TitleLot Number
      Anti-Diasialoganglioside GD3, -Q2810272 Q2810272
      Anti-Diasialoganglioside GD3, clone R24 - 3383088 3383088
      Anti-Diasialoganglioside GD3, clone R24 - 3905435 3905435
      Anti-Diasialoganglioside GD3, clone R24 - 4083431 4083431
      Anti-Diasialoganglioside GD3, clone R24 - 4138370 4138370
      Anti-Diasialoganglioside GD3, clone R24 - 4194981 4194981
      Anti-Diasialoganglioside GD3, clone R24 - 4264850 4264850