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MAB1072 Anti-Erythropoietin Antibody, clone 1C8

Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.

Anti-Erythropoietin Antibody, clone 1C8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: Metabolic Hormones & Receptors Gene Symbol: EPO, MGC138142, EP UniProt Number: P01588
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MAB1072
250 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, IF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1072
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Erythropoietin Antibody, clone 1C8
      Alternate Names
      • erythropoietin
      • Epoetin
      • epoetin
      Background InformationErythropoietin (UniProt: P01588; also known as Epoetin, EPO) is encoded the EPO gene (Gene ID: 2056) in human. EPO is an acidic glycoprotein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off in the endoplasmic reticulum and the mature protein undergoes glycosylation in the Golgi prior to its release. EPO is mainly produced by peritubular fibroblasts in the renal cortex and to some extent by the liver in adult mammals. In fetal and neonatal mammals, it produced in the liver. EPO is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia. It is induced by ischemic preconditioning by activation of (HIF)-1alpha. It is reported to contribute to angiogenesis in response to ischemia through upregulation of VEGF/VEGFR. It binds to EPO receptor that leads to receptor dimerization and JAK2 activation that results in activation of specific downstream effectors, including STAT1 and STAT3. Endogenous EPO consists of three N-linked polysaccharide groups and one O-linked group. Although its glycosylation slightly reduces its biological activity but is shown to be essential for ensuring its prolonged circulation in the plasma. The N-glycans in its structure protect it from the action of proteases and in the modulation of its receptor binding affinity. Mutations in EPO gene can cause microvascular complications in type II diabetic subjects and erythrocytosis. (Ref.: Bunn, HF (2013). Cold Spring Harb. Perspect. Med. 3(3); a011619).
      References
      Product Information
      FormatPurified
      Control
      • Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
      PresentationPurified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 300 mM NaCl, and 0.02% sodium merthiolate.
      Quality LevelMQ100
      Applications
      ApplicationDetect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Western Blotting
      Application NotesELISA:
      For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.

      Immunosorbent preparation

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant human Epo.
      Clone1C8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with human Erythropoietin (Epo).
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
      Gene Symbol
      • EPO
      • MGC138142
      • EP
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01588 # Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
      SIZE: 193 amino acids; 21307 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
      SIMILARITY: SwissProt: P01588 ## Belongs to the EPO/TPO family.
      Molecular Weight34 kDa (glycosylated)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Mouse kidney lysates.

      Western Blot Analysis:
      1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1072 04053252266386

      Documentation

      Anti-Erythropoietin Antibody, clone 1C8 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Erythropoietin Antibody, clone 1C8 Certificates of Analysis

      TitleLot Number
      Anti-Erythropoietin, clone 1C8 - 2121751 2121751
      Anti-Erythropoietin, clone 1C8 - 2148240 2148240
      Anti-Erythropoietin, clone 1C8 - 2111385 2111385
      Anti-Erythropoietin, clone 1C8 - 2197223 2197223
      Anti-Erythropoietin, clone 1C8 - 2327716 2327716
      Anti-Erythropoietin, clone 1C8 - 2330589 2330589
      Anti-Erythropoietin, clone 1C8 - 3184765 3184765
      Anti-Erythropoietin, clone 1C8 - 3207103 3207103
      Anti-Erythropoietin, clone 1C8 - 4082572 4082572
      Anti-Erythropoietin, clone 1C8 - LV1514199 LV1514199
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies