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MABE1898-25UL Anti-FUS (TLS) Antibody, Clone 10F7

Anti-FUS (TLS), Clone 10F7, Cat. No. MABE1898, is a mouse monoclonal antibody that detects RNA-binding protein FUS and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

MABE1898-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1898-25UL
      DescriptionAnti-FUS (TLS) Antibody, Clone 10F7
      Alternate Names
      • RNA-binding protein FUS
      • 75 kDa DNA-pairing protein
      • Oncogene FUS
      • Oncogene TLS
      • POMp75
      • Translocated in liposarcoma protein
      Background InformationRNA-binding protein FUS (UniProt: P35637; also known as 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein) is encoded by the FUS (also known as TLS) gene (Gene ID: 2521) in human. Fused in sarcoma/translocated in liposarcoma FUS/TLS is a multi-functional nuclear protein that binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in super helical double-stranded DNA. It plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. It may also play a role in maintenance of genomic integrity. It binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. FUS is reported to be involved in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. A chromosomal aberration involving FUS is shown to cause of acute myeloid leukemia (AML). Mutations in FUS gene have been linked to Amyotrophic lateral sclerosis 6, with or without frontotemporal dementia that affects upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. (Ref.: Lagier-Tourenne, C., et al. (2010). Hum. Mol. Genet. 19(1); R46-R64).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-FUS (TLS), Clone 10F7, Cat. No. MABE1898, is a mouse monoclonal antibody that detects RNA-binding protein FUS and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected FUS (TLS) in HeLa cell lysate.

      Western Blotting Analysis: A representative lot detected FUS (TLS) in Western Blotting applications (Zhou, Y., et. al. (2013). PLoS Genet. 9(10):e1003895).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected FUS (TLS) in Chromatin Immunoprecipitation applications (Zhou, Y., et. al. (2013). PLoS Genet. 9(10):e1003895).

      Immunoprecipitation Analysis: A representative lot detected FUS (TLS) in Immunoprecipitation applications (Zhou, Y., et. al. (2013). PLoS Genet. 9(10):e1003895).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged full-length recombinant human FUS protein.
      Clone10F7
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 10F7 is a mouse monoclonal antibody that detects RNA binding protein FUS.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FUS
      • TLS
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~65 kDa observed; 53.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected FUS (TLS) in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1898-25UL 04061842196272