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MAB2617 Anti-Kindlin-2 Antibody, clone 3A3

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MAB2617
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, BWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2617
      DescriptionAnti-Kindlin-2 Antibody, clone 3A3
      Alternate Names
      • Mitogen-inducible gene 2 protein
      • Pleckstrin homology domain-containing family C member 1
      • fermitin family homolog 2
      • kindlin 2
      • mitogen inducible gene 2 protein
      Background InformationThe Kindlin family is a group of focal adhesion proteins that have become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. The isoform Kindlin-2 specifically participates in the recruitment of migfilin protein to focal adhesion sites for cell-ECM interactions, coordinates the manufacture of actin and modifications to cell shape. It is also actively involved in connecting ECM adhesion sites and the actin cytoskeleton (Shi, 2007). Kindlin-2 is pervasively expressed throughout tissue.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Kindlin-2 Antibody, clone 3A3 is validated for use in WB, IC for the detection of Kindlin-2.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Kindlin-2.
      EpitopeUnknown
      Clone3A3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody recognizes Kindlin-2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with bovine based on 100% sequence homology.
      Mouse and rat (98% sequence homology).
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FERMT2
      • KIND2
      • Kindlin-2
      • MIG-2
      • MIG2
      • PLEKHC1
      • UNC112
      • UNC112B
      • kindlin-2
      • mig-2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.

      SUBUNIT STRUCTURE: Interacts with FBLP1.

      SUBCELLULAR LOCATION: Cytoplasm › cell cortex. Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Note: Within actin stress fibers at cell-ECM focal adhesion sites.

      TISSUE SPECIFICTY: Ubiquitous. Found in numerous tumor tissues.

      INDUCTION: By serum, in lung fetal fibroblast cultured cells.

      DOMAIN: The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.

      SEQUENCE SIMILARITIES: Belongs to the kindlin family.

      Contains 1 FERM domain.

      Contains 1 PH domain.
      Molecular Weight~ 70 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected Kindlin-2 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2617 04053252615429

      Documentation

      Anti-Kindlin-2 Antibody, clone 3A3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Kindlin-2 Antibody, clone 3A3 Certificates of Analysis

      TitleLot Number
      Anti-Kindlin-2, clone 3A3 2484231
      Anti-Kindlin-2, clone 3A3 3169486
      Anti-Kindlin-2, clone 3A3 - 2360015 2360015
      Anti-Kindlin-2, clone 3A3 - 2463631 2463631
      Anti-Kindlin-2, clone 3A3 - 1944087 1944087
      Anti-Kindlin-2, clone 3A3 - 1966924 1966924
      Anti-Kindlin-2, clone 3A3 - 1997496 1997496
      Anti-Kindlin-2, clone 3A3 - 2038285 2038285
      Anti-Kindlin-2, clone 3A3 - 2108580 2108580
      Anti-Kindlin-2, clone 3A3 - 2153090 2153090

      References

      Reference overviewPub Med ID
      Integrin α3β1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt.
      Yazlovitskaya, EM; Tseng, HY; Viquez, O; Tu, T; Mernaugh, G; McKee, KK; Riggins, K; Quaranta, V; Pathak, A; Carter, BD; Yurchenco, P; Sonnenberg, A; Böttcher, RT; Pozzi, A; Zent, R
      Molecular biology of the cell  26  1857-74  2015

      Show Abstract
      25808491 25808491