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ABS94 Anti-MLK1 Antibody

Anti-MLK1 Antibody detects level of MLK1 & has been published & validated for use in IH(P).

Anti-MLK1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: MAP3K9, MLK1, PRKE1 UniProt Number: P80192
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ABS94
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Chp, Rhesus MacaqueIH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS94
      DescriptionAnti-MLK1 Antibody
      Alternate Names
      • Mitogen-activated protein kinase kinase kinase 9
      • Mixed lineage kinase 1
      Background InformationMLK1 (MAP3K9) is a serine–threonine kinase of the Mixed-Lineage Kinase (MLK) family capable of activating the c-Jun N-terminal Kinase (JNK)/Mitogen-Activated Protein Kinase (MAPK) pathway and regulating the other two prinicpal MAPK cascades, p38 and extracellular signal regulated kinase (ERK). Immunoblots and mass spectrometry show MLK is threonine phosphorylated in or near the activation loop. Studies show that MLKs play a role in the TGF-beta1-induced cell death mechanism.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Colorectal adenocarcinoma tissue
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MLK1 Antibody detects level of MLK1 & has been published & validated for use in IH(P).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human MLK1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Chimpanzee
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with Human.
      Predicted to react with Rat, Mouse, Chimpanzee, and Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAP3K9
      • MLK1
      • PRKE1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Activates the JUN N-terminal pathway.

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      COFACTOR: Magnesium.

      ENZYME REGULATION: Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation (By similarity).

      SUBUNIT STRUCTURE: Homodimer (By similarity).

      TISSUE SPECIFICITY: Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.

      PTM: Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

      Contains 1 protein kinase domain.

      Contains 1 SH3 domain.

      SEQUENCE CAUTION: The sequence AAI11408.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight121 kDa (isoform 1), 123 kDa (isoform 2) calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in colorectal adenocarcinoma tissue.

      Immunohistochemistry Analysis: A 1:300 dilution of this antibody detected MLK1 in colorectal adenocarcinoma tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS94 04053252592096